Laboratory Operations Flashcards

1
Q

Describe the principle of the following testing technique:

Spectrophotometry

A

Used for qualitative & quantitative measurement of the light properties of biological & chemical compounds as a function of wavelength.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Visible light ranges from violet to red between what wavelengths?

A

380 to 750 nm.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What device converts light into a measurable electrical signal?

A

Photodetector.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Describe the principle of the following testing technique:

Atomic Absorption Spectrophotometry

A

A spectroanalytical method used for the quantitative determination of chemical elements by using the absorption of specific frequencies of light by free metallic ions or atoms.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Describe the principle of the following testing technique:

Osmometry

A

Measures the concentration of solute particles (electrolytes, proteins, organic compounds) that contribute to the osmotic pressure of a solution.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Describe the principle of the following testing technique:

Nephelometry & Turbidimetry

A

Analytic techniques to measure light scattered by particles.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Gaussian distribution refers to…

A

a distribution of data symmetrically around the mean, with most values closest to the center.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Systematic error is a result of…

A

Inaccuracy.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Random error is a result of…

A

Imprecision.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Describe accuracy.

A

The extent to which a test result approximates the “true” value.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Describe precision.

A

The reproducibility of a result.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Describe sensitivity.

A

The ability of the test to detect disease when present.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Describe specificity.

A

The ability of the test to detect only the disease sought.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

How would a reference interval be established?

A
How well did you know this?
1
Not at all
2
3
4
5
Perfectly