Laboratory Operations Flashcards
Describe the principle of the following testing technique:
Spectrophotometry
Used for qualitative & quantitative measurement of the light properties of biological & chemical compounds as a function of wavelength.
Visible light ranges from violet to red between what wavelengths?
380 to 750 nm.
What device converts light into a measurable electrical signal?
Photodetector.
Describe the principle of the following testing technique:
Atomic Absorption Spectrophotometry
A spectroanalytical method used for the quantitative determination of chemical elements by using the absorption of specific frequencies of light by free metallic ions or atoms.
Describe the principle of the following testing technique:
Osmometry
Measures the concentration of solute particles (electrolytes, proteins, organic compounds) that contribute to the osmotic pressure of a solution.
Describe the principle of the following testing technique:
Nephelometry & Turbidimetry
Analytic techniques to measure light scattered by particles.
Gaussian distribution refers to…
a distribution of data symmetrically around the mean, with most values closest to the center.
Systematic error is a result of…
Inaccuracy.
Random error is a result of…
Imprecision.
Describe accuracy.
The extent to which a test result approximates the “true” value.
Describe precision.
The reproducibility of a result.
Describe sensitivity.
The ability of the test to detect disease when present.
Describe specificity.
The ability of the test to detect only the disease sought.
How would a reference interval be established?
