(1) General Chemistry & QA

Question 1

Recognize the following category of glassware and its use:

Kimax/Pyrex-borosilicate

Answer 1

All purpose uses; high thermal resistance.

Question 2

Recognize the following category of glassware and its use:

Corex-aluminosilicate

Answer 2

Centrifuge tubes and thermometers; resists scratching; high thermal resistance.

Question 3

Recognize the following category of glassware and its use:

Vycor

Answer 3

Acid and alkaline resistant; high thermal resistance.

Question 4

Recognize the following category of glassware and its use:

High silica

Answer 4

Cuvettes and thermometers, optically pure; high temperature changes.

Question 5

Recognize the following category of glassware and its use:

Low actinic

Answer 5

Ambered color.

Question 6

Recognize the following category of glassware and its use:

Flint-soda lime

Answer 6

Disposable.

Question 7

Explain the significance of an etched ring near the top of a glass pipette.

Answer 7

It signifies that the pipette must be blown out after sample delivery in order to get the full volume.

Question 8

Describe the types and uses of the following measuring and transfer pipette:

Mohr

Answer 8

Graduations do not extend to the tip, not a blow out.

Question 9

Describe the types and uses of the following measuring and transfer pipette:

Serological

Answer 9

Graduations throughout the stem of the pipette, blow out-etched ring.

Question 10

Describe the types and uses of the following measuring and transfer pipette:

Micropipette

Answer 10

Volume < 1 mL.

Question 11

Describe the types and uses of the following measuring and transfer pipette:

Volumetric

Answer 11

Bulblike enlargement in the stem, single volume delivery; not a blow out.

Question 12

Describe the types and uses of the following measuring and transfer pipette:

Ostwald-Folin

Answer 12

Larger bore for viscous fluids, blow out-etched rings.

Question 13

Describe the types and uses of the following measuring and transfer pipette:

Measuring or graduated

Answer 13

Dispense several different volumes and used repeatedly for a solution; used for transferring reagents and making dilutions.

Question 14

Describe the types and uses of the following measuring and transfer pipette:

Transfer

Answer 14

Dispense one volume; greatest degree of accuracy and precision, used for diluting standards, calibrators, patient samples, or quality control material.

Question 15

Describe TC pipettes.

Answer 15

greatest accuracy, are designed to contain an exact fixed amount of solution. They are rinsed out.

Question 16

Describe TD pipettes.

Answer 16

delivers an exact amount. They do not rinse out.

Question 17

At what temperature should pipettes be calibrated?

Answer 17

20 - 22C.

Question 18

Describe the designation for manufactured glassware including pipettes.

Answer 18

Class A specifications are defined by NIST ensuring the most accurate volume of liquid delivery.

Question 19

Discuss calibration of automated pipettes.

Answer 19

• Non-Class A pipettes must be verified by calibration.
• Gravimetric procedure where 1 mL of water = 1 g at 4C.
• Pipettes are checked quarterly at room temperature by weighing the volume of water delivered by the pipette.

Question 20

What type of micropipette uses a piston device to facilitate aspiration and ejection of liquids with disposable tips that are changed with use?

Answer 20

Air Displacement Micropipette.

Question 21

What type of micropipette uses a capillary tip made of glass or plastic with a Teflon-tipped plunger to transfer liquid?

Answer 21

Positive Displacement Micropipette.

Question 22

What is the cleaning method of glassware for heavy metals or molecular assays?

Answer 22

Acid washed glass using dichromate and nitric acid.

Final rinse with reagent grade water.

Question 23

List the common types of resins used in plastic ware i the clinical laboratory and their uses.

Answer 23

Polystyrene, polyethylene, polypropylene, Tygon, Teflon, polycarbonate, polyvinyl chloride-tubing.