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NT - Lab - 309 > Laboratory Equipment > Flashcards

Laboratory Equipment Flashcards

1
Q

What is the function of the Microscope?

A

Enable vision of elements that are too small to be seen with the naked eye

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2
Q

Microscopy

A

Inspection with magnification

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3
Q

Macroscopy

A

Gross inspection with the naked eye

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4
Q

What objects can be identified with microscopy? - CELLS

A

CELLS Red Blood Cells White Blood Cells Sperm Platelets Looking at ratio and identification

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5
Q

What objects can be identidied with microscopy? - URINE

A

URINE Bacteria Casts Crystals

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6
Q

What objects can be identified with microscopy? PARASITES

A

PARASITES Worm eggs Lice Mites

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7
Q

Microscope elements Eye Piece

A

MONOCULAR BINOCULAR TRINOCULAR Eye piece holds the occular lense. Magnification of x10

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8
Q

Microscope elements Nose Piece

A

Lower end of the body tube Holds the objective lenses Rotate clockwise - LOWER - HIGHER

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9
Q

Microscope elements Objective Lenses

A

Housed in the nose pieces x4 - scanning x10 - low power x40 - high dry x100 - oil immersion

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10
Q

Total magnification

A

Objective lens x Eye piece e.g. 40 x 10 = x400

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11
Q

Microscope elements Limb / Arm

A

Connects the base with the body Supports the stage and condenser

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12
Q

Microscope elements Stage

A

A flat platform oh which the slide is placed. A hole in the centre allows light from the condenser to illuminate the specimen Stage can be moved up and down by the course and fine adjustment knobs.

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13
Q

Microscope elements

Mechanical stage

A

Attached to the stage, holds the slide in place as well as allowing movement of the slide up and down and side to side by using the mechanical stage hand wheel.

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14
Q

Microscope elements

Vernier scales

A

Scales that allows the relocation of a certain point of the slide.

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15
Q

Microscope element

Substage condenser

A

Below the stage Consists of two lenses that condense the light from the light source onto the specimen to make it brighter and the image sharper.

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16
Q

Microscope elements

Iris diaphragm

A

adjusting the aperture of the iris diaphragm with the control can regulate the amount of the light that passes through the condenser

17
Q

Microscope elements

Base

A

Houses the light source houses on/off switch

18
Q

Microscope elements

Rheostat

A

changes the intensity of the light

19
Q

Battlement technique

A

the technique of looking at the body of a slide thoroughly. Start left-hand side

20
Q

Define - Supernatant

A

Fluid from a spun sample

21
Q

Define - Sediment

A

Solid from a spun sample

22
Q

Functions of a centrifuge

A

Seperates sample Serum - cells from fluid PCV - cells of different densities Urine - concentrating material (sediment)

23
Q

Spinning speeds Blood

A

10,000 RPM for 5 mins

24
Q

Spinning speeds Urine

A

2,000 RPM for 5 mins

25
Q

Refractometers

A

Refractometers measure the degree to which the light refracts in the device. The concentration of the liquid changes how much refraction occurs. Should be calibrated before every use

26
Q

Dry chemistry blood analysers

A

Slide impregnated with chemicals, sample dropped onto slide Read + Interpret colour change

27
Q

Wetchemistry blood analysers

A

Small containers that have ‘wet’ chemicals in. Uses chemical reaction to determin level in the sample

28
Q

Spectrophotometer

A

mechanically measures colour change within a sample colour change represents the levels within a sample

29
Q

Quality assurance

A

overall programme which ensures final results reported by the laboratory are all correct

30
Q

Quality control

A

the measures in place to ensure the test is working correctly to ensure accuracy, precision and reliability. Manufacturers will provide a sample with known values to asses any inconsistensies

31
Q

Glassware

A

Petri Dishes Test tubes conicle flasks slides/coverslips PVC tubes - haemocrit tubes

NT - Lab - 309 (3 decks)

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