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Bacteriology > Laboratory 1 > Flashcards

Laboratory 1 Flashcards

1
Q

what method is used for sampling bacteria on skin

A

swab method

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2
Q

what method is used for sampling bacteria on water

A

spread method

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3
Q

substances with microorganisms that are introduced in culture media for purpose of initiating growth or causing specific reaction

A

innoculum

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4
Q

exposure of a petri dish w/ sterile NA on an open space or area

A

Ubiquity

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5
Q

agar liquefies at what temperature

A

100C

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6
Q

Agar solidifies at what temperature

A

40C

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7
Q

population of cells come from one microbial species; only one species of microbes present

A

Pure culture

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8
Q

Multiple distinct species of microbes; colonies of different sizes and shapes

A

Mixed culture

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9
Q

Different form of colonies; do not mix or blend

A

Contamination

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10
Q

petri dish that contains agar

A

Culture media

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11
Q

Nutrients prepared for microbial growth

A

Culture medium

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12
Q

No presence of microbes

A

Sterile

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13
Q

Introduction of microbes onto medium

A

Inoculum

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14
Q

Microbes that grown on medium

A

Culture

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15
Q

Agar is made up of what

A

Complex polysaccharide

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16
Q

Complete removal of microbes

A

Sterilization

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17
Q

Uses moist heat to destroy all microbes

A

Autoclave

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18
Q

Killing or inhibiting vegetative microbes

A

Disinfection

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19
Q

Antimicrobial chemicals safe for skin

A

Antiseptics

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20
Q

microbes grow at room temperature

A

Mesophile

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21
Q

Requires oxygen to grow

A

Aerobic

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22
Q

Used to create an anaerobic environment

A

Gaspak

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23
Q

What does a candle jar help with

A

Reducing oxygen

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24
Q

Give examples pf general growth media

A

1 nutrient agar
2 trypticase soy agar

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25
Q

Give examples of differential media

A

MacConkey agar
Ethylene-methylene blue
Shigella salmonella agar

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26
Q

Large growth of microbes; branching; extends outward the inoculation point

A

Swarming

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27
Q

Tiny/pinpoint size; appear on colonies

A

Punctiform

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28
Q

Mass or cluster; various sizes and colors

A

Colony

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29
Q

This prevents contamination of microbes, of self, and of work area

A

Aseptic technique

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30
Q

substances or mixtures that is used to cultivate microbes; provide needed nutrients, ph, balance, and others to support growth of microbes

A

Media

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31
Q

Which type of media is used to observe large quantity of microbes?

A

Liquid media

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32
Q

agar on petri dish

A

Solid media

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33
Q

Agar on test tubes

A

Liquid media

34
Q

Type of media that encourage the growth of certain types of microorganisms while inhibiting the growth of others

A

Selective media

35
Q

Type of media that encourage the growth of certain types of microorganisms while inhibiting the growth of others

A

Selective media

36
Q

Type of media that is formulated to show visible differences between different types of microorganisms based on their growth characteristics or biochemical reactions

A

Differential media

37
Q

is a liquid medium that supports microbial growth and is commonly used for studying bacteria in liquid culture; grow cells in small space

A

Broth

38
Q

Shows bacterial turbidity

A

Broth

39
Q

solidified at an angle, providing a larger surface for microbial growth, often used for organisms requiring increased oxygen exposure; for investigating the presence of swarming

A

Slant

40
Q

solidified at an angle, providing a larger surface for microbial growth, often used for organisms requiring increased oxygen exposure; for investigating the presence of swarming

A

Slant

41
Q

Used for long term storage

A

Slant

42
Q

solidified vertically, suit anaerobic organisms, and are used to observe specific growth patterns

A

Deep

43
Q

Used to observe bacterial motility

A

Deep

44
Q

Petri dishes with solid agar surfaces are used for microbial isolation, forming distinct colonies

A

Plates

45
Q

Used for microbiological tests like susceptibility test

A

Plates

46
Q

is a versatile medium for growing various microorganisms like bacteria, yeast, and molds. It contains essential nutrients

A

Nutrient agar

47
Q

What is in a nutrient media/agar?

A

Peptone, beef extract, and agar

48
Q

a thin or thick membrane of bacterial growth that spreads across the surface and appears floating on the liquid broth

A

Pellicle

49
Q

small chunks of cells floating in the liquid

A

Flocculent

50
Q

growth on the bottom

A

Sediment

51
Q

What indicates the presence of microbial growth in a broth culture?

A

Turbidity

52
Q

These are patterns of growth on what type of solid media:

  1. arborescent (branched)
  2. beaded
  3. echinulate (pointed)
  4. filiform (even)
  5. rhizoid (rootlike)
  6. spreading
A

Slant

53
Q

True or false: Loops are preferable in deep culture

A

False. Needles are preferable in deep culture

54
Q

What equipment is used to transfer bacteria in slant

A

Inoculating loop

55
Q

The following growth patterns can be observed in what type of media:

  • crateriform
  • napiform
  • infundibuliform
  • saccate
  • stratiform
A

Deep

56
Q

The following growth patterns can be observed in what type of media:

  • uniform fine turbidity
  • flocculant growth
  • pellicle
  • sediment
A

Broth

57
Q

a steam sterilizer used to sterilize lab equipment at 121 Celsius or 249 Fahrenheit

A

Autoclave

58
Q

Used to transfer a culture of microorganisms from point A to point B by streaking the media

A

Inoculating loop

59
Q

Used to transfer a culture of microorganisms by stabbing the media

A

Inoculating needle

60
Q

Insulated, electrically heated cabinet meant for providing organisms with optimum temperature for growth

A

Incubator

61
Q

can operate at temperatures up to 300C and has a fan for circulating hot air. used for sterilization of glassware and materials that are spoiled by moist heat

A

Hot air oven

62
Q

used for heating and melting of media, solutions, samples etc. at temperatures below 100C

A

Boiling water bath

63
Q

used for sterilizing inoculating loop, plating out cultures, transferring cultures, heat-fixing of smears and creating a sterile zone for
aseptic operation

A

Bunsen burner

64
Q

used in microbiology laboratory for storing / preserving cultures, media, and many sensitive materials

A

Refrigerator

65
Q

used when working with hazardous or toxic chemicals to make sure they are contained

A

Biosafety cabinet

66
Q

used for preserving moisture-sensitive specimen

A

Dessicator

67
Q

Organisms in this level typically do not cause disease in healthy humans, and therefore do not pose a risk to humans. No special containment is required.

A

BSL-1

68
Q

Organisms at this level pose moderate health risks to humans and may cause disease; physical barriers needed if working with aerosols or splashing.

A

BSL-2

69
Q

Give examples of BSL-2 organisms

A

Salmonella enterica
Staphylococcus aureus
Streptococcus pyogenes

70
Q

This level contains airborne organisms which pose a more serious or lethal risk to humans; requires special containment and barrier equipment, as well as ventilation systems; laboratory access is restricted.

A

BSL-3

71
Q

Give examples of BSL-3 organisms

A

Bacillus anthracis
Mycobacterium tuberculosis

72
Q

Contains organisms which pose a lethal risk to humans; there are no vaccines or available treatment; strict safety precautions and containment; use of self-contained “space suits”

A

BSL-4

73
Q

Give examples of BSL-4 organisms

A

Ebola
Smallpox

74
Q

Used for chemical preparation; ventilated; traps and exhausts vapors, gasses, and nanoparticles

A

Fumehood

75
Q

Enclosed work station to create contamination-free work; protect from dust and other airborne contaminatiom

A

Laminar flow hood

76
Q

Used for magnification of microbes

A

Microscope

77
Q

Uses ULPA filter

A

BSL-3

78
Q

Uses UV and HEPA filter

A

BSL-2

79
Q

What percentage of alcohol is antiseptic for the skin

A

70% alcohol

80
Q

What percentage of alcohol is used for disinfecting glasswares

A

90-95% alcohol

81
Q

What equipment is used in spread plate

A

L-shaped spreader (rod)

Bacteriology (4 decks)

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