Laboratoires Flashcards
Nutrient agar
permissive medium
aerobic plate count
AIA
Actinomycetes selective
Sabouraud
fungus (selective)
Azo
Azotobacter (selective, no nitrogen source)
KEA
Enterococci, esculin degraders turns the medium black
Endo
Coliforms, lactose fermenters turn red
EMB
Coliforms, lactose fermenters: blue-black
mFC
Coliforms, lactose fermenters: blue
Endo, EMB, mFc
Coliforms, lactose fermenters
RCAF
Propionibacterium pink or buff colored colonies
4S
Staphylococcus black colonies plus white precipitate: areus
Snyder agar
production of lactic acid : yellow
3 main groups of microorganisms in soil
1 bacteria
2 actinomycete
3 funfi
Nessler s reagent test for…
color…
presence of ammonia
turns yellow-brown
the darker the color, the more ammonia
Azotobacter vinelandii : aerobe or anaerobe
aerobe
Clostridium spp. : aerobe or anaerobe
anaerobe
Describe Clostridia (3)
1- medium-sized
2- Gram positive
3- rods with central to sub-terminal endospores
Susceptibility to dental caries
what test
Snyder
propionibacteria : aerobe, anaerobe, fac aerobe… ?
aerotolerant anaerobe
propionibacteria inhabit what part of your skin
hair canals
micrococci aerobe or anaerobe
aerobe
RCAF
how does P. acnes looks like
buff-coloured colonies
RCAF
how does P.avidum looks like
pink and mucoid
RCAF
how does P. granulosum looks like
pink and dry
what organisms can be dangerous if you are a carrier and you work in a hospital
S. aureus
4 s agar to determine …
if you are a staph carrier
positive S. aureus
what color
Blue granules
For which test you had to chew parafilm
Snyder
major milk borne disease (3)
1- tuberculosis
2- brucellosis
3- typhoid
What is flash pasteurization
heat to 71 C
15 seconds
nitrite prevents growth of
C. botulinum
TGEA
aerobic count plates
EMB definition
Levine Eosin Methylene Blue agar
KEA definition
Kanamycin Esculin Azide agar
4S definition
Single step Selection for Staphylococci