Food microbiology Flashcards
HTST
high t, short time
Why canning?
increase shelf life at room temperature
How to can acidic food?
T: 100 C
How to can non acidic food?
autoclave: 121 C
What is 12D process?
10^12 spores of Clostridium botulinum killed
How to take out water to inhibit growth?
- sun dry
- hot air drying
- freeze-drying (lyophilization) result in little lost of quality but is really expensive
Irradiation
Radappertization
kill ALL microorganism
Irradiation
Radicidation
kill SPECIFIC microorganisms
Irradiation
Radurization
reduce overall contamination
Purpose of nitrites
prevent growth of clostridium botulinum
present in celery extract (natural)
Purpose of sulfites
-wine industry
=inhibits wild yeast
What produces Nisin
lactococcus lactis
What is a primary metabolite
a metabolite produced during exponential growth
What is a secondary metabolite
produced when microorganisms are in the stationary phase
-competition
Is a fermentor like a chemostat?
YES
What does a fermentor control? (4)
- temperature
- nutrients
- pH
- dissolved oxygen
Gmo to produce 2 things (that we learned)
Insulin
rennet (baby cows)
Staphylococus areus : endotoxin or exotoxin
exotoxin
food poisoning
E. Coli : endotoxin or exotoxin
endotoxin
infection
Salmonellosis : food poisoning or infection
infection
endotoxin
C botulinum in adults : infection of food poisoning
food poisoning
C botulinum in infants : infection of food poisoning
infection
What type of yeast to produce a good beer?
Saccharomyces cerevisiae
In fermented products, what are the types of microorganisms the most often used?
lactic acid bacteria
yeasts
Define Lactic acid bacteria (4 characteristics)
- GRAM +
- AEROTOLERANT ANAEROBES
- Rods or cocci
- homofermentative or heterofermentative
Homofermentative and heterofermentative both produce
Lactic acid
Homofermentative (some food)
pickles,
sauerkraut = choucroutte
Heterofermentative (some food)
Sausages (Salami)
fermented milk products
Name 2 yogurt cultures
Streptococcus thermophilus
Lactobacillus bulgaris
Curdling of cheese : what does precipitate the cheese to make a curd?
Lactic acid bacteria
Ripening of cheese : what adds flavor?
lactic acid bacteria and other organisms
What cleaves casein?
Lactic acid bacteria or rennin
Principal steps to make cheese (5)
1 add starter culture 2 add rennin or lactic acid bacteria 3 whey is drained off 4 heat it, not eat it 5 add salt (optionnal)
Cheese ripening
Cheddar : what bacteria do we add
lactic acid bacteria
Cheese ripening
Swiss cheese : what bacteria do we add for holes
how much time required for ripenning?
Propionibacteria
3-12 months or longer