Lab6: Enzymes Activity Flashcards
What is the main characteristics of enzymes?
They are catalysts
What is a catalase?
An antioxidant enzyme that catalyzes hydrogen peroxide (h2O2)
Why does the enzyme accelerate the rate of the reaction?
Because they lower the activation energy
What does catalase have of special that allows the induced fit?
An active site where H2O2 can bind
Write down the chemical breakdown of catalase and hydrogen peroxide
2 H2O2 + catalase — 2 H2O2 + O2 + catalase
what are three environmental conditions that could have an effect on enzymes?
-temperature
-salinity
-pH
What does the temperature affect on enzymatic activity?
Affect all bonds
What is the effect pH and salinity?
Introduce ions disturbing ionic bonds between R groups
Each enzymes as optimal conditions and they are based on what?
Their 3D structure
Why does the filter paper lift up to the surface during the experiment?
Because the O2 produced get stuck in the filter paper
What are the positive and negative control of the experiment?
Positive control: enzyme + water at room temperature
Negative control: water at room temperature
What is the effect of a high amount of cell?
Decrease catalase activity
Why is it important to maintain a stable pH?
To have optimal enzyme function
Why if the effect of a very high pH or a very low pH?
It affects the structure and the function of the enzyme
How do we know that an enzyme is saturated?
When it reached is maximum velocity (*see the graph)
Why is it important to have a negative control during the experiment?
For validating results, to exclude contaminants, to state if our conclusion drawn is reliable or not
