Lab4: Fermentation Flashcards
What type of cells are baker’s yeast (saccharomyces cerevisiae)
Sigle celled eukaryote
What lyophilised mean?
Dried under vacuum and cold temperatures
What is an aerobic respiration?
When there is presence of oxygen
What is an anaerobic respiration pathway?
When there is no oxygen — fermentation — recycling NAD+
Why yeast cell are facultative anaerobes?
Because they could do aerobic respiration but prefer anaerobic alcoholic fermentation
What is the equation for the metabolism of glucose?
C6H12O6 — 2CO2 + 2 C2H5OH
Reactant — waste + product
Glucose — carbon hydroxide + Ethanol
Steps of glucose metabolism?
-Glucose
-Pyruvic acid
-Acetaldehyde
-Ethanol
Why NBM (nitrogen base medium) does react?
Because it doesn’t contain sugar
Why does the galactose doesn’t react?
Because they dont have transporter — can’t hydrolysed — can’t enter the cell
Why does this type of yeast can’t breakdown galactose?
because it doesn’t contant the enzymes to break it down
What product is released during glucose fermentation?
CO2
How many molecules of carbon dioxide is produced for every molecule of glucose that is metabolized by fermentation to ethanol?
2
What would be the indépendant and dépendant variable for this experiment?
Indépendant: the type of sugar
Dépendent: the rate of CO2 production (ml/min)
