Lab2: Microscopes Flashcards
What are the two main points of handling procedure of microscopes?
-always carry in an upright position
-Always set at the lowest magnification,position,stage when living
What is the important step before changing magnification?
Center the part of the specimen
What are the five MAJOR aspects to represent data in a table?
-Title
-Column header
-Column titles
-Row header
-Row titles
What is the approximated diameter of the cheek cell?
50 mium
What are the two things to label on the cheek cell?
-plasma membrane
-nucleus
What is the must important rule for biological drawing?
DRAW ONLY WHAT YOU SEE
What is important not to forget in biological drawing?
To write down the total magnification
What is the simple formula to calculate the total magnification?
Product of ocular lens + objective lens
If the ocular lens is of 4.5X and the objective lens of 10X, what would be the total magnification?
45X
At what magnification it is very important to NOT use a ruler?
10X and 40X
What type of ruler do we use to calculate the field of view?
Transparent ruler
What is the resulting of increasing magnification and leaving the lens there for a while?
The lens could dry
What are the two main step for calculating the field of view?
1-Measure the diameter of the field, then find the radius
2-Calculate the are of the field with the equation (2peiR)
What type of variables are kept constant during the experiment?
Controlled variables
What is the tentative of of explanation for an observation?
Hypothesis
What is the variable that the investigator varies in the experiment?
Indépendant variables
What is the process used to measure the dépendant variable?
Procedure
What is the appropriate value to use for thé indépendant variable?
Level of treatment
What is the treatment that eliminates the independent variable or sets it at a standard level?
Control
What the investigator counts, measures, records; it is being affected in the experiment?
Dépendant variable
The number of times the experiment is repeated?
Replication
A statement of the expected results in an experiment based on the hypothesis
Prediction
Conversion method: if the total magnification is equal to 400X, what is it equal to in ocular micrometer constant?
2,5 o.u
If the total magnification is of 400X and you want to calculate the total number of micrometer, what would you do? And what would be the answer here?
400X = 2.5 o.u
4.0 o.u x 2.5 = 10 micrometers
What are the two types of cell that require a wet-mount?
-Euglena
-Paramecium
What are the main step to prepare a wet-mount?
1-put your specimen on the glass
2-put a stain of the dilute you need
3-place the cover over your small solution
4-observe the changes threw the movement of the cell
What change do you need to do on your microscope to use an ocular micrometer?
-remove the ocular from the microscope
-replace the ocular lens with the ocular micrometer
1 millimeter is equal to how much micrometer?
1000 micrometers
