Lab1 Flashcards
1
Q
Where were the DNA isolated from?
A
AML patients
2
Q
Why are lysates loaded on silica membrane? Washing step?
A
To adsorb DNA and remover protein and contaminants
Residual contaminants
3
Q
What does buffer AL and PBS do?
Ethanol?
Buffer AE?
A
Lysate
Precipitates the proteins and lipids
Maximizes DNA yield
4
Q
What should you store DNA in and why not water?
A
Buffer AE, because there might be acid hydrolysis. AE stabilizes it longer and is not acidic.
5
Q
What are the main steps of PCR? What’s the point of it?
A
Denaturation, annealing, primer
extensions in many cycles. Amplifies target DNA
6
Q
What is in the master mix?
A
Reaction buffer, 2 primers, taq polymerase. Dntp, template, water
