DNA array

Question 1

Outline the general concepts of a microarray

Answer 1

It anlayzes 1000s of genes, proteins, RNA on silicon chip

Question 2

Briefly cover how microarrays are produced

Answer 2

Pins deposit probes on silicon chips that are fluorescently labelledwhere the sample DNA/Mrna/ protein will hybridize to it, if that specific sequence is present and will emit fluorescence when excited with specific wavelength of lasers, it captured and quantified.

Question 3

highlight the some of the capabilities and problems associated with MAs

Answer 3

+: Can detect a large number of genes at the same time. Can analyze mRNA, proteins,
Automated, high throughput
-: Not vary good in detecting small changes or chromatin changes, has to have a good sample
Has to be supported with other data, can’t make conclusions based off it

Question 4

What are the sort of things we can study with MAs

Answer 4

Assessing chromosome amplification, deletion
Transcription factor binding
Single nucelotide polymorphism
Expression profiling
Translational activity 
Pathogen identification
Protein arrays