Lab techniques

Question 1

DNA sample cleaved into small pieces and electrophoresed & transferred to filter

Answer 1

southern blot

Question 2

RNA sample cleaved & electrophoresed & transferred to filter

Answer 2

northern blot

Question 3

sample protein is separated via gel electrophoresis & transferred to filter

Answer 3

western blot

Question 4

confirmatory test for HIV after pos. ELISA

Answer 4

western blot

Question 5

identifies DNA binding proteins (ie. TF’s) using labeled oligonucleotide probes

Answer 5

southwestern blot

Question 6

profiles gene expression levels of thousands of genes simult, detects single nt polymorphisms, copy # variations

Answer 6

microarrays

Question 7

pt blood sample probed using a test Ab to see if specific Ag is present

Answer 7

direct ELISA

Question 8

pt blood sample probed using either a test Ag to see if specific Ab is present
uses secondary Ab coupled to color generating enzyme

Answer 8

indirect ELISA

Question 9

fluorescent DNA or RNA probe binds specific gene site of interest on chrom used for specific localization of genes & direct visualization of anomalies at molecular level

Answer 9

fluorescence in situ hybridization

Question 10

inducibly manipulate genes at specific developmental points ie. to study a gene deletion that causes embryonic death

Answer 10

cre-lox system

Question 11

dsRNA made compl to mRNA sequence of interest - transfected into human cells - dsRNA separates & promotes degradation of target mRNA

Answer 11

RNA interference