Lab techniques

Question 1

What is PCR useful for?

Answer 1

A technique that can amplify small amounts of DNA and is useful in modern applications such as sequencing or determining cellular mRNA levels

Question 2

Western blot

Answer 2

Western blot measures protein levels in a sample using antibodies

Question 3

Southern blot

Answer 3

This technique detects DNA levels.

Question 4

Northern Blot

Answer 4

This technique detects RNA levels

Question 5

Gel electrophoresis

Answer 5

This technique separates molecules on the basis of size

Small molecules move fastest

(DNA is negatively charged)

Question 6

SDS-Page and reducing gels

Answer 6

Separates denatured proteins on the basis of size

Large substances elute first

Question 7

Native gels

Answer 7

Separates proteins in their native conformation that allows subunits to remain intact

Question 8

Molecular cloning

Answer 8

Used to introduce genetic information into a plasmid for protein expression or genetic manipulation

Question 9

Transformation/conjugation/transduction

Answer 9

Used to transfer genetic information into bacterial species