Lab Technics

Question 0

Differential stain

Answer 0

Differentiates 2 common types of bacteria by using a Gram Stain
1st is crystal violet that stains +
2nd stain is safranin red or pink-
Differentiates two common types of bacteria

Question 1

Brightfield microscope

Answer 1

Light microscope

Limited to 2000x resolution

Question 2

Bacteria sizes

Answer 2

1-4 microns

1000 microns=1 millimeter

Question 3

100x oil immersion objective lens

Answer 3

Is used in concert with a 10x eyepiece totaling a magnification of 1000x

Question 4

Typical compound light microscope

Answer 4

Ocular ( eyepiece) lens magnify 10x what the objective lens already magnifies 10x ( low power) 40x ( high power) or 100x ( oil immersion)
Condenser adjust contrast
Focus is course or fine

Question 5

Stains

Answer 5

Dye used to improve contrast

Methylene Blue
Crystal Violet Purple hue

Question 6

Gram stain

Answer 6

Is widely used in clinical microbiology
Uses 2 stains
1 crystal violet and stains gram-+
2 safranin is red or pink gram -

Question 7

Differential Stain

Answer 7

Differentiates
Gram -
Gram + bacteria

Endospore / Acid-fast
These are used to visualize spore-forming bacteria ( anthrax) ( TB)

Question 8

Electron Microscope

Answer 8

No light powerful used to see viruses 0.03-0.3 microns

Use heavy metal and electron beams to resolve images of viruses

Question 9

Transmission electron microscope (TEM)

Answer 9

Electrons pass through the specimen leaves dark areas that reflect the outline the viral outline and used to view internal structures of any specimen at a 100,000x magnification

Question 10

Scanning Electron Microscope (SEM)

Answer 10

Scans an object and reconstructs it by computer technology

Question 11

Medium

Answer 11

A nutritious extract or mixture of material that will support the growth of microbes

Question 12

Tryptic Soy Agar

Tryptic Soy Broth

Answer 12

(TSA) and (TSB)

Most common medium