Lab techinques

Question 1

Procedure used to amplify a desired fragment of DNA

Answer 1

Polymerase chain reaction

Question 2

Gel electrophoresis and tagging of DNA

Answer 2

Southern blot

Question 3

Gel electrophoresis and tagging of RNA

Answer 3

Northen blot

Question 4

Gel electrophoresis and tagging of protein

Answer 4

Western blot

Question 5

Gel electrophoresis and tagging of DNA-binding protein

Answer 5

Southwestern blot

Question 6

Laboratory technique to assess size, granularity and protein expression of individual cells in a sample

Answer 6

Flow cytometry

Question 7

Detects the relative amount of complementary binding of DNA or RNA probes to nucleic acid sequences arrenged in grids on glass or silicon

Answer 7

Microarrays

Question 8

Detect SNPs and CopyNumberVariations

Answer 8

Microarrays

Question 9

Immunologic test used to detect the presence of either a specific antigen or antibody

Answer 9

Enzyme-linked immunoabsorbent assay

Question 10

Speficity: Western blot vs Enzyme-Linked immunoabsorbent array

Answer 10

Western blot >ELISA

Question 11

A process in which metaphase chromosomes are stained, ordered and numbered according to morphology, size, arm-lenght ratio and banding pattern

Answer 11

Karyotyping

Question 12

FISH: Fluorescence in situ hybridization

Answer 12

Fluorescent DNA or RNA probe binds to specific gene site of interest on chromosomes

Question 13

Cre-lox system use

Answer 13

Manipulate genes at specific developmental points

Question 14

RNA interference

Answer 14

dsRNA is synthesized that is complementary to the mRNA sequence of interest, eliminating it, knocking out gene expression