LAB: SPECIMEN CULTURE AND PROCESSING Flashcards
Fungi culture should be incubated at what temperature?
25C or 30C
Rapidly growing fungi
Mucor spp and Rhizopus spp
Slow growing fungi
Fonsecaea spp or Phialopora spp
Gentamicin and Chloramphenicol inhibits _______ growth
Bacterial growth
Cycloheximide inhibits the growth of _____________
Bacteria and environmental fungi
It is a type of PDA that is recommended for microbial enumeration of yeast and molds for cosmetics
PDA supplemented with chlortetracycline
It is a type of PDA that is recommended for the selective cultivation of fungi from mixed samples
PDA supplemented with chloramphenicol
It is a type of culture media that is recommended for the isolation and enumeration of yeast and molds
Potato Dextrose Broth
It is an enriched, non-selective media used for isolating and cultivating various bacteria, including yeasts and molds.
Brain Heart Infusion agar
Specimen processing for TINEA
Direct Microscopy: KOH wet mount and Calcofluor-stained mount
Culture: SDA w/ chloramphenicol & gentamicin, incubate at 26C
Specimen processing for CANDIDA
Direct Microscopy: KOH wet mount and Calcofluor-stained mount
Culture: SDA w/ chloramphenicol & gentamicin, incubate at 35C
Ratio for Biphasic Filter technique
1:10 or 1:20
Blood to broth ratio
A superior method compared to biphasic filter technique, specimens are treated with Triton-X and Sodium carbonate to lyse blood cells
Membrane Filter Technique
It is recommended for patients with suspected fungal septicemia
Lysis Centrifugation Isolator System
Stain essentials for direct microscopy of Tissue Biopsy
Hematoxylin and Eosin stain
Grocott’s Methenamine Silver stain
Periodic acid-Schiff stain
