LAB: SPECIMEN COLLECTION

Question 1

Fungi lives as ___________ in nature and are capable of life cycle with or without human host

Answer 1

SAPROPHYTES

Question 2

this apparatus should be used to reduce personnel exposure to fungal elements

Answer 2

CLASS II BIOLOGICAL SAFETY CABINET

Question 3

Between culture and microscopic examination which is the priority if the specimen is not sufficient

Answer 3

CULTURE should be the priority if the specimen is insufficent

Question 4

What is the specimen needed for patients suspected for Tinea or Ringworm in the Hair and Scalp

Answer 4

Scrapings from the infected area

Question 5

What is the specimen needed for the infection in the Skin

Answer 5

Skin scraping and place in KOH wet mount

Question 6

It is a handheld device that emits long wave ultraviolet light that assists in identifying fungi-infected skin, nail, hair and scalp

Answer 6

Wood’s lamp

Question 7

What is the wavelength used for Wood’s lamp device

Answer 7

340-365nm

Question 8

How many mL of blood should be collected for the diagnosis of fungal septicemia?

Answer 8

10mL for smears preparation
3-5mL must be centrifuged for direct culture

Question 9

Most common specie recovered in the blood

Answer 9

Candida spp

Question 10

Optimal temperature for blood fungal culture

Answer 10

30C and must be maintained for 4 weeks

Question 11

How many mL of CSF is needed for fungal investigation

Answer 11

3-5mL of CSF

Question 12

Storage temperature for CSF when processing is delayed

Answer 12

Room temperature or incubate at 30C

Question 13

This fungal element is destroyed in grinding process

Answer 13

Zygomycete

Question 14

Best time of collection for fungi that originated in the lungs

Answer 14

Morning collection

Question 15

24-hour samples from the lungs are unaccepted for what reason?

Answer 15

samples may be overgrown with bacteria and fungal contaminants

Question 16

Specimen storage temperature for respiratory and urine specimen

Answer 16

4C