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Hematology > [LAB] Specimen Collection > Flashcards

[LAB] Specimen Collection Flashcards

1
Q

Enumerate the order of draw.

A

Yellow - sterile
Light blue - sodium citrate
Red - no additives
Green - heparin
Lavender - EDTA
Gray - sodium fluoride or sodium oxalate

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2
Q

What is the usual gauge size of a needle when collecting blood from an adult?

A

21-23

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3
Q

What is the usual length of a needle when collecting blood using a syringe?

A

1-1.5in

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4
Q

What is the method of collection when collecting blood from pediatric or geriatric populations?

A

Winged blood collection
“butterfly”

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5
Q

What is the usual length of a needle when collecting blood using winged blood collection?

A

0.5-0.75in

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6
Q

What is the length range of the plastic tubing used in the butterfly collection?

A

5-12in

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7
Q

What is the result of a prolonged tourniquet application?

A

hemolysis and hemoconcentration

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8
Q

What is the minimum time of tourniquet application before reapplying it?

A

2mins

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9
Q

During the collection of blood, what is the maximum time for tourniquet application to avoid hemoconcentration and hemolysis?

A

1min

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10
Q

What vein has a high likelihood of hematoma formation?

A

Basilic vein

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11
Q

What vein is located on the thumb side of the arm?

A

Cephalic vein

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12
Q

What is the vein of choice in venipuncture?

A

Median Cubital Vein

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13
Q

What refers to the preferred site for venipuncture?

A

Antecubital fossa

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14
Q

What is the appropriate way of anchoring the vein?

A

L-holding technique

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15
Q

What is the angle of the needle in venipuncture?

A

15-30 degree angle

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16
Q

What is the recommended method of blood collection from young children?

A

Capillary puncture

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17
Q

Area for a capillary puncture on infants.

A

Lateral surface of the plantar side of the heel

18
Q

Area for a capillary puncture on children who started walking.

A

Plantar surface of big toe

19
Q

Area for a capillary puncture in adults.

A

The palmar surface of the distal or end segment of the middle or ring finger of non-dominant hand

20
Q

How deep is the puncture during capillary puncture?

A

less than 2mm

21
Q

Sites with cyanotic appearance indicates what?

A

decrease blood flow

22
Q

Enumerate the features of a well-made peripheral blood smear.

A
  1. 2/3 to 3/4 length of the slide
  2. finger-shaped film
  3. visible lateral edges
  4. smooth film
  5. rainbow appearance in the feather edge
23
Q

What is the stain used in staining the blood smears?

A

Wright-giemsa

24
Q

Enumerate the components of Wright-Giemsa.

A
  1. Methanol
  2. Methylene blue
  3. Eosin
  4. Sodium phosphate or aged distilled water
25
Q

What is the pH of sodium phosphate?

A

6.4

26
Q

What is the pH range of aged distilled water?

A

6.4-6.8

27
Q

What refers to the technique when examining the peripheral blood smear?

A

battlement pattern

28
Q

What is the color of the peripheral blood smear macroscopically?

A

pink to purple

29
Q

At what part of the smear will you look when observing the cell morphology under OIO?

A

feather edge of the slide

30
Q

What is the color of the erythrocyte under OIO?

A

orange to salmon pink

31
Q

What is the time interval wherein will you check for a blood clot in the clotting time test?

A

every 30 seconds

32
Q

What is the normal reference range for clotting time procedure?

A

8-15 minutes

33
Q

What is the normal reference range for a bleeding time procedures?

A

2-4 minutes

34
Q

What is the depth of puncture when doing bleeding time procedure?

A

3mm

35
Q

What is the time interval wherein will you blot the filter paper with blood for a bleeding time procedure?

A

every 30 seconds

36
Q

What is the size of the blood drop in the slide when doing blood smear preparation?

A

1-2mm diameter

37
Q

What is the angle of the spreader slide when doing blood smear preparation?

A

30-40 degree

38
Q

What will you do when you put too much blood for the blood smear?

A

Raise the angle of spreader

39
Q

What will you do when you put too little blood for the blood smear?

A

Lower the angle of the spreader

40
Q

What will you do during blood smear preparation when the patient has a low hematocrit level?

A

Raise the angle of the spreader

Hematology (8 decks)

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