[LAB] Specimen Collection Flashcards
Enumerate the order of draw.
Yellow - sterile
Light blue - sodium citrate
Red - no additives
Green - heparin
Lavender - EDTA
Gray - sodium fluoride or sodium oxalate
What is the usual gauge size of a needle when collecting blood from an adult?
21-23
What is the usual length of a needle when collecting blood using a syringe?
1-1.5in
What is the method of collection when collecting blood from pediatric or geriatric populations?
Winged blood collection
“butterfly”
What is the usual length of a needle when collecting blood using winged blood collection?
0.5-0.75in
What is the length range of the plastic tubing used in the butterfly collection?
5-12in
What is the result of a prolonged tourniquet application?
hemolysis and hemoconcentration
What is the minimum time of tourniquet application before reapplying it?
2mins
During the collection of blood, what is the maximum time for tourniquet application to avoid hemoconcentration and hemolysis?
1min
What vein has a high likelihood of hematoma formation?
Basilic vein
What vein is located on the thumb side of the arm?
Cephalic vein
What is the vein of choice in venipuncture?
Median Cubital Vein
What refers to the preferred site for venipuncture?
Antecubital fossa
What is the appropriate way of anchoring the vein?
L-holding technique
What is the angle of the needle in venipuncture?
15-30 degree angle
What is the recommended method of blood collection from young children?
Capillary puncture
Area for a capillary puncture on infants.
Lateral surface of the plantar side of the heel
Area for a capillary puncture on children who started walking.
Plantar surface of big toe
Area for a capillary puncture in adults.
The palmar surface of the distal or end segment of the middle or ring finger of non-dominant hand
How deep is the puncture during capillary puncture?
less than 2mm
Sites with cyanotic appearance indicates what?
decrease blood flow
Enumerate the features of a well-made peripheral blood smear.
- 2/3 to 3/4 length of the slide
- finger-shaped film
- visible lateral edges
- smooth film
- rainbow appearance in the feather edge
What is the stain used in staining the blood smears?
Wright-giemsa
Enumerate the components of Wright-Giemsa.
- Methanol
- Methylene blue
- Eosin
- Sodium phosphate or aged distilled water
What is the pH of sodium phosphate?
6.4
What is the pH range of aged distilled water?
6.4-6.8
What refers to the technique when examining the peripheral blood smear?
battlement pattern
What is the color of the peripheral blood smear macroscopically?
pink to purple
At what part of the smear will you look when observing the cell morphology under OIO?
feather edge of the slide
What is the color of the erythrocyte under OIO?
orange to salmon pink
What is the time interval wherein will you check for a blood clot in the clotting time test?
every 30 seconds
What is the normal reference range for clotting time procedure?
8-15 minutes
What is the normal reference range for a bleeding time procedures?
2-4 minutes
What is the depth of puncture when doing bleeding time procedure?
3mm
What is the time interval wherein will you blot the filter paper with blood for a bleeding time procedure?
every 30 seconds
What is the size of the blood drop in the slide when doing blood smear preparation?
1-2mm diameter
What is the angle of the spreader slide when doing blood smear preparation?
30-40 degree
What will you do when you put too much blood for the blood smear?
Raise the angle of spreader
What will you do when you put too little blood for the blood smear?
Lower the angle of the spreader
What will you do during blood smear preparation when the patient has a low hematocrit level?
Raise the angle of the spreader
