Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Hematology > [LAB] Hemostasis Lab Testing (Part 2) > Flashcards

[LAB] Hemostasis Lab Testing (Part 2) Flashcards

1
Q

What test is designed to detect qualitative platelet abnormalities and is used on patients with symptoms of mucocutaneous bleeding?

A

Platelet function test

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

How much platelet count indicates the presence of bleeding symptoms?

A

> 50,000/µL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q
  • “obsolete”
  • original test of platelet function
A

Bleeding time

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Reference interval of bleeding time.

A

2-9min.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What affects the bleeding time procedure?

A
  • intracapillary pressure
  • skin thickness
  • size and depth of the wound
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q
  • high complexity test
  • used to detect platelet adhesion, aggregation, and secretion
A

platelet aggregometry

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

How much PRP will you add to the cuvette in the platelet aggregometry procedure?

A

500µL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

How many minutes will you incubate the PRP in platelet aggregometry at 37℃?

A

5min.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

True or False.

As platelet aggregates form, more light passes through the PRP.

A

True (↑ light transmittance)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What is the principle used in whole blood platelet aggregometry?

A

electric impedance

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What is the principle used in PRP platelet aggregometry?

A

light-transmittance

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is the dilution ratio for specimen and normal saline on whole blood platelet aggregometry?

A

1:1

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Suspension volume range used on whole blood platelet aggregometry.

A

300-500µL

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

True or False.

The rise in impedance is directly proportional to platelet aggregation.

A

True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What happens when platelets adhere to the electrodes and one another during aggregation on whole blood platelet aggregometry?

A

it will impede the current

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What aggregometry is used for the simultaneous measurement of platelet aggregation and secretion of ATP from activated platelet granules?

A

Platelet lumiaggregometry

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

How many aliquots of the sample will you use in platelet lumiaggregometry?

A

2 aliquots

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What will you add to the first sample during platelet lumiaggregometry?

A

ATP and luciferin-luciferase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

What will you add to the second sample during platelet lumiaggregometry?

A

agonist and luciferin-luciferase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Identify the VWF Assay.

  • reflect VWF activity rather than concentration and offer improved precision when compared to the VWF: Rco
A

Manual ELISA

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

Identify the VWF Assay.

  • uses monoclonal antibody specific for an active VWF epitope
A

VWF activity immunoassay

22
Q

Identify the VWF Assay.

  • mimics VWF’s in vivo collagen adhesion property
A

VWF collagen binding assay

23
Q
  • Aggregation assay that measures platelet activation and secretion induced by HIT Ab in the presence of heparin.
  • reference and confirmatory method for HIT
A

carbon 14 serotonin release assay (C-SRA)

24
Q

What is the agonist used in aggregation-based tests?

A

Unfractionated heparin (UFH)

25
Q

What indicates the ↑ plasma level in PF4?

A

thrombotic stroke or coronary thrombosis

26
Q

What is used in PF4 immunoassays to prevent in vitro platelet activation?

A

CTAD tubes

27
Q

Identify the platelet activation marker.

  • an active product of the eicosanoid pathway
A

thromboxane A2

28
Q

Identify the platelet activation marker.

  • stable, measurable plasma metabolite from Thromboxane A2
A

Thromboxane B2

29
Q

Identify the platelet activation marker.

  • used to characterize in vivo platelet activation
  • uses a random urine sample
  • maybe used to monitor aspirin therapy and to identify cases of aspirin therapy failure
A

11-dehydrothromboxane B2

30
Q

What is the first in vitro clot procedure?

A

Lee-White whole blood coagulation time test

31
Q

What is modified version of Lee-White procedure that uses a particulate clot activator in the test tube that speeds the clotting process?

A

Activated clotting time (ACT) test

32
Q

What test is used as a point-of-care assay to monitor UFH therapy in high-dose applications?

A

Activated clotting time (ACT) test

33
Q

Enumerate the components of PT reagents (thromboplastin).

A
  • affinity-purified tissue factor
  • phospholipids
  • buffered 0.025M calcium chloride (CaCl2)
34
Q

What is the specimen used for prothrombin time assay?

A

platelet-poor plasma (PPP)

35
Q

The volume of PPP used in PT assay.

A

50 or 100mL

36
Q

Premeasured volume of reagent used in PT assay.

A

100 or 200mL

37
Q

PT reference range.

A

12.6-14.6 seconds

38
Q

True or False.

Reporting PT assays should be at nearest tenth of second.

A

True

39
Q
  • used to monitor the effects of UFH
  • detect LAC-specific coagulation factor antibodies
A

Partial Thromboplastin Time

40
Q

Enumerate PTT reagent components.

A
  • phospholipid
  • negatively charged particulate activators
41
Q

Reference interval of PTT.

A

26-38 seconds

42
Q

Typical therapeutic range when monitoring heparin therapy with PTT.

A

60-100 seconds

43
Q

Identify the anticoagulant.

  • nonspecific inhibitor used in PTT mixing studies.
  • signals a potential thrombotic risk
A

Lupus anticoagulant

44
Q

What assay uses the principle whereby it cleaves fibrinopeptides A and B from plasma fibrinogen to form detectable fibrin polymer?

A

Thrombin Clotting time

45
Q

How much thrombin is transferred into the PPP aliquot during thrombin clotting time?

A

200mL

46
Q

Reference interval for thrombin clotting time.

A

15-20 seconds

47
Q
  • isolated from the venom of Bothrops atrox
  • catalyzes the conversion of fibrinogen to fibrin
A

Reptilase

48
Q

Reptilase only cleaves _____ from the ends of the fibrinogen molecule.

A

fibrinopeptide A

49
Q
  • an assay that is useful for detecting hypofibrinogenemia and dysfibrinogenemia
A

Reptilase time assay

50
Q
  • from the Daboia russelii viper that triggers factor X
  • “Stypven time”
A

Russell Viper Venom

Hematology (6 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions