[LAB] Routine Stool Examination Flashcards
BRIGHT RED STOOL IS INDICATIVE OF WHAT
LOWER GIT BLEEDING
BEETS
TOMATOES
FOOD COLORING
USE OF FOBT
SUSPECTED CASES OF GASTROINTESTINAL DISEASES
FOBT IS USED AS A SCREENING PROCEDURE OF
COLORECTAL CANCER
AMOUND OF BLOOD IN STOOL FOR IT TO BE CONSIDERED AS PATHOLOGICALLY SIGNIFICANT
2.5 ML
150 G
VERY SMALL AMOUNT OF BLOOD THAT IS NOT VISIBLE TO THE NAKED EYE
OCCULT BLOOD
HOW IS OCCULT BLOOD DETECTED
CHEMICAL MEANS ONLY
GENERAL PRINCIPLE OF FOBT
PSEUDOPEROXIDASE ACTIVITY
HEMOGLOBIN DECOMPOSES HYDROGEN PEROXIDE INTO WATER AND OXYGEN
LIBERATED OXYGEN OXIDIZES A CHROMOGEN INTO A COLORED PRODUCT
ORDER OF CHROMOGEN SENSITIVITY
MOST TO LEAST
BENZIDINE
O-TOLUIDINE
GUAIAC
PREFERRED CHROMOGEN FOR FOBT
GUAIAC
WHY IS GUAIAC PREFERRED
DETECTS CLINICALLY SIGNIFICANT AMOUNTS OF BLOOD
DOES NOT REACT TO THE MINIMAL AMOUNT OF BLOOD NORMALLY PRESENT IN A STOOL SAMPLE
EXAMPLE OF A COMMERCIAL KIT FOR OCCULT BLOOD TESTING
HEMASCREEN LAB PACK
EATING THESE 3 DAYS PRIOR TO SPECIMEN COLLECTION CAUSES FALSE POSITIVE RESULTS
RED, RARE MEAT
HORSERADISH, MELONS, CANTALOUPE, RAW BROCOLLI
SUBSTANCES TO AVOID 7 DAYS PRIOR TO SPECIMEN COLLECTION TO PREVENT FALSE POSITIVES
ASPIRIN
NSAIDS
STRONG REDUCING AGENTS THAT CAUSE FALSE NEGATIVE RESULTS
VITAMIN C (>250 mg/day)
IRON SUPPLEMENTS
CAUSES OF FALSE POSITIVES
MENSTRUAL BLOOD
HEMORRHOID CONTAMINATION
RED MEAT
ASPIRIN
NSAIDS
TWO METHODS FOR MICROSCOPIC EXAMINATION
DIRECT FECAL SMEAR
KATO THICK SMEAR
REAGENTS USED IN DFS
NORMAL SALINE SOLUTION
LUGOL’S IODINE
D’ANTONI’S IODINE SOLUTION
BUFFERED METHYLENE BLUE
STORAGE CONTAINER OF D’ANTONI’S IODINE SOLUTION
AMBER
GLASS-STOPPERED OR SCREW CAPPED BOTTLE
ROOM TEMPERATURE
TRUE OR FALSE
LIGHT INFECTIONS CANNOT BE DETECTED IN DFS
TRUE
WHAT KIND OF MOUNT IS USED FOR DETECTING MOTILE TROPHOZOITES
SALINE MOUNTS
WHAT ARE PROTOZOAN CYSTS DEMONSTRATED AS IN DFS
REFRACTILE BODIES
STAINING COLOR OF CYTOPLASM IN DFS
YELLOW
SOLUTIONS STORED IN AMBER BOTTLES
LUGOL’S IODINE
D’ ANTONI’S IODINE SOLUTION
DILUTING RATION IN LUGOL’S IODINE
1:5
LUGOL’S IODINE TO DISTILLED WATER
SOLUTION STORED IN CLEAR BOTTLES
NORMAL SALINE SOLUTION
WHO INTRODUCED THE KATO THICK SMEAR
KATO AND MIURA IN 1954
AMOUNT OF STOOL NEEDED IN KTS
ABOUT 50 MG STOOL
SIZE OF 2 MONGGO BEANS
KTS CLEARING SOLUTION
GLYCEROL
COLORING SOLUTION IN KTS
MALACHITE GREEEN
OTHER NAME OF KTS
CELLOPHANE-COVERED THICK SMEAR
SIZE OF CELLOPHANE STRIP
22x30 mm
SOAKING SOLUTION FOR CELLOPHANE IN KTS
GLYCEROL-MALACHITE GREEN SOLUTION
FOR HOW LONG IS THE CELLOPHANE STRIP SOAKED IN GMG SOLUTION
24 HOURS PRIOR TO USE
HOW LONG IS THE CELLOPHANE AND MALACHITE GREEN PREPARATION LEFT BEFORE EXAMINATION
20-30 MINUTES AT ROOM TEMPERATRURE
TIME LIMIT FOR KTS EXAMINATION
1 HOUR AFTER PREPARATION
METHOD USED FOR MASS SURVEY
KTS
METHOD THAT IS GOOD AT DETECTING EGGS WITH THICK SHELLS
KTS
LIMITATION OF KTS
NOT USED FOR PROTOZOA OR HELMINTH LARVAE
[FORMED ELEMENTS]
SLOUGHING
EPITHELIAL CELLS