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PARASITOLOGY > [LAB] Permanent Stains > Flashcards

[LAB] Permanent Stains Flashcards

1
Q

[COMPONENTS]
IODINE

A

LUGOL’S SOLUTION
- 100mL Distilled Water
- 10g Potassium Iodide
- 5g Iodine Crystals

GRAM’S IODINE
- 1 part Lugol’s Solution
- 14 parts Distilled Water

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2
Q

[COMPONENTS]
LUGOL’S SOLUTION

A
  • 100mL Distilled Water
  • 10g Potassium Iodide
  • 5g Iodine Crystals
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3
Q

[COMPONENTS]
GRAM’S IODINE

A
  • 1 part Lugol’s Solution
  • 14 parts Distilled Water
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4
Q

[COMPONENTS]
MODIFIED D’ ANTONI’S IODINE

A

-100 mL Distilled Water
- 1g Potassium Iodide
- 1.5g Powdered Iodine Crystals

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5
Q

STORAGE CONDITION OF MODIFIED D’ ANTONI’S IODINE

A

STORE IN BROWN BOTTLE, AWAY FROM LIGHT

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6
Q

TIME WHEN D’ ANTONI’S IODINE CAN BE USED AFTER PREPARATION

A

4 DAYS

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7
Q

[COMPONENTS]
MERTHIOLATE IODINE FORMALDEHYDE

A
  • 0.10mL Lugol’s Solution
  • 0.125mL Formaldehyde
  • 0.774 mL Tincture of Merthiolate
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8
Q

TECHNIQUE OF WHEATLEY

A

TRICHROME STAIN

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9
Q

MODIFICATION OF GOMORI’S STAINING

A

TRICHROME STAIN

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10
Q

[COMPONENTS]
TRICHROME STAIN

A
  • 0.6g Chromotrope 2R
  • 0.3g Light Green SF
  • 0.7g Phosphotungstic Acid
  • 1mL Glacial Acetic Acid
  • 100mL Distilled Water
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11
Q

[RESULTS]
TRICHROME STAIN
CYTOPLASM

A

BLUE-GREEN
WITH A TINGE OF PURPLE

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12
Q

[RESULTS]
TRICHROME STAIN
NUCLEI AND INCLUSIONS

A

SLIGHTLY MORE PURPLE

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13
Q

[RESULTS]
TRICHROME STAIN
BACKGROUND MATERIAL

A

GREEN

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14
Q

[STAIN]
CYTOPLASM IS STAINED BLUE-GREEN WITH A TINGE OF PURPLE

A

TRICHROME STAIN

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15
Q

[STAIN]
NUCLEI AND INCLUSIONS ARE COLORED RED

A

TRICHROME STAIN

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16
Q

[STAIN]
CYST IS STAINED SLIGHTLY MORE PURPLE

A

TRICHROME STAIN

17
Q

[STAIN]
BACKGROUND AND MATERIALS ARE STAINED GREEN

A

TRICHROME STAIN

18
Q

[COMPONENTS]
IRON HEMATOXYLIN STAIN
MODIFIED SPENCER MONROE METHOD

A

STOCK SOLUTION
- 10g Hematoxylin Crystals
-100 mL 95% Ethanol

WORKING SOLUTION
- 1 part Hematoxylin Stock Solution
- 19 parts distilled Water

19
Q

OTHER TERM FOR THE IRON HEMATOXYLIN STAINC

A

MODIFIED SPENCER MONROE METHOD

20
Q

OTHER TERM FOR MODIFIED SPENCER MONROE METHOD

A

IRON HEMATOXYLIN STAIN

21
Q

[RESULTS]
IRON HEMATOXYLIN STAIN
BACKGROUND

A

BLUE GRAY

22
Q

[RESULTS]
IRON HEMATOXYLIN STAIN
CELLS AND ORGANISMS

A

VARIOUS INTENSITIES OF BLUE-GRAY

23
Q

[RESULTS]
IRON HEMATOXYLIN STAIN
INCLUSIONS, CHROMATOIDAL BODIES, AND NUCLEAR STRUCTURES

A

DARKER THAN THE SURROUNDING CYTOPLASM

24
Q

[STAIN]
BACKGROUND MATERIAL IS STAINED BLUE-GRAY

A

IRON HEMATOXYLIN STAIN
MODIFIED SPENCER-MONROE METHOD

25
Q

[STAIN]
CELLS AND ORGANISMS ARE STAINED VARIOUS INTENSITIES OF BLUE GRAY

A

IRON HEMATOXYLIN STAIN
MODIFIED SPENCER-MONROE METHOD

26
Q

[STAIN]
INCLUSIONS, CHROMATOIDAL BODIES, AND NUCLEAR STRUCTURES ARE STAINED DARKER THAN THE SURROUNDING CYTOPLASM

A

IRON HEMATOXYLIN STAIN
MODIFIED SPENCER-MONROE METHOD

27
Q

WHAT DOES CALCOFLUOR WHITE STAIN

A

ACANTHAMOEBA CYSTS

28
Q

[COMPONENTS]
CALCOFLUOR WHITE

A

DISODIUM SALT OF 4,4-BIS-(4-ANILINO-BIS-DIETHYLAMINO-5-TRIAZIN-2-YLAMINO)-2,2-ATILBENE DISULFONIC ACID

29
Q

NON SPECIFIC FLUOROSCENT DYE

A

CALCOFLUOR WHITE

30
Q

[STAINS]
ACANTHAMOEBA CYSTS

A

CALCOFLUOR WHITE

31
Q

[RESULTS]
ACANTHAMOEBA CYST STAINED WITH CALCOFLUOR WHITE

A

DOUBLE WALLED
OUTER WALL IS WRINKLED
CYCTS WILL FLUORESCE

32
Q

NEGATIVE CONTROL OF CALCOFLUOR WHITE

A

E.coli WILL NOT FLUORESCE
MOST BACTERIA AND OTHER DEBRIS WILL NOT FLUORESCE
SOME YEAST AND DEBRIS MAY

33
Q

SPECIAL STAINS FOR MICROSPORIDIA

A

MODIFIED TRICHROME-WEBER GREEN
MODIFIED TRICHROME-RYAN BLUE

34
Q

[STAINS]
PENETRATES THE SPORES OF THE PARASITES

A

MODIFIED TRICHROME-WEBER GREEN
MODIFIED TRICHROME-RYAN BLUE

35
Q

SPECIAL STAINS FOR COCCIDIA

A

MODIFIED KINYOUN’S ACID-FAST STAIN (COLD)
MODIFIED ZIEHL-NEELSEN ACID-FAST STAIN (HOT)

36
Q

[STAIN]
COLD METHOD

A

MODIFIED KINYOUN’S ACID FAST STAIN

37
Q

[STAIN]
HOT METHOD

A

MODIFIED ZIEHL-NEELSEN ACID FAST STAIN

38
Q

[COMPONENTS]
KINYOUN’S CARBOL FUCHSIN

A
  • 4g Basic Fuchsin
  • 8g Liquified Phenol
  • 20mL 95% Ethanol
  • 100mL Distilled Water
39
Q

[COMPONENTS]
LOEFFLER’S ALKALINE METHYLENE BLUE

A
  • 0.3g Methylene Blue
  • 30mL 95% Ethanol
  • 100mL KOH
  • Sulfuric Acid (Decolorizer)

PARASITOLOGY (5 decks)

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