LAB RELATED QUESTIONS Flashcards
Identify 4 good readons why fruit flies are favorite organisms for genetic studies
- males and females have distinct appearances
- produce large # of offspring
- short generation time (10-20 days)
- virgin females can be obtained for control mating
- can be easily anesthetized and manipulated
Properly spell the 2 word scientific name of fruit flies used this semester:
Drosophila MelanOgaster
Know the difference between the larval and pupal stage of the fruit fly.
Larval: White, segmented, wormlike. Larva eats continuously and grows continuously.
Pupal stage: is immobike, nonfeeding stage
The darker the older
Know the lifecycle if the fruitfly
-feeding larval stage (three subdivision)
- first instar
-second instar
-third instar
On the third the third instar molts one more time to form: PUPA
THEN: metamorphosis
ECLOSION–> emergence of adult
Know the phenotypes of the mutants studied
Mutations White - white eyes Sepia -brown eyes Apterous- no wings Ebony- dark pigment Vestigial- small wings Scarlet- red eyes Brown- brown eyes Antennapedia
Mutations
Wildtype
(W) white mutant white eyes
(+) wildtype
Antennapedia
Legs in place of antennae
Apterous
No wings
Ebony
Dark pigment
Vestigial
Small wings
Be able to tell the sex of the fruitfly given an image of it
Male- ROUND TIP Dark genital hair Female- pointy tip - no dark genitals -no sex combs
Understand pteridine differences and similarities among various mutants used
Eye pigments of drosophila
Pteridines —> colorful, soluable in alcohol/ammonia
Ommoonomes—> brown, insoluable in alcohol/ ammonia
X^+X^w
Red eyes
Chi-Square test
Observed- given Expected is (204) (1/4)= 104
Expected is answer after multiplied by (3/4)
Plug into equation
Independent assortment
Dihybrid
