Lab Quiz 3

Question 1

What are neutrophils, eosinophils, basophils, lymphocytes, and monocytes?

Answer 1

They are all white blood cells

Question 2

Which of the following is not a granulocyte? Check all that apply.

Answer 2

monocyte
lymphocyte

Question 3

The largest cells in the blood that leave the bloodstream to become macrophages are the:

Answer 3

monocytes

Question 4

Match the following leukocytes with their relative abundance.

Answer 4

Neutrophils 55-56%

Eosinophils 1-3%

Basophils 0.2%

Lymphocytes 25-33%

Monocytes 3-7%

Question 5

Match the following WBCs with their function.

Answer 5

phyagocytosis, mostly bacteria neutrophils

attack parasites eosinophils

sustain inflammatory response by releasing heparin and histamine basophils

active in specific acquired immunity Lymphocytes

phagocytosis and antigen presentation monocytes

Question 6

PMNs are another name for:

Answer 6

neutrophils

Question 7

Mature neutrophils are also called segs because the nucleus is segmented into 2-5 lobes Immature neutrophils are also called Immature neutrophils are also called bands because the nucleus lacks segmentation.

Answer 7

Mature neutrophils are also called segs because the nucleus is segmented into 2-5 lobes Immature neutrophils are also called Immature neutrophils are also called bands because the nucleus lacks segmentation.

Question 8

Hematopoiesis occurs in which of the following?

Answer 8

bone marrow

Question 9

Match the following UV radiation to their corresponding wavelengths.

Answer 9

UV-A 315-400 nm

UV-B 280-315 nm

UV-C 100-280 nm

Question 10

Rank the following microorganisms from the least resistant (1) to the most resistant (4):

Answer 10

1 G+ bacteria

2 G- bacteria

3 mycobacteria

4 endospores

Question 11

Which bacteria do we use for the UV radiation experiment? Check all that apply.

Answer 11

B. subtilis

E. coli

Question 12

Which bacterium do you expect to be more resistant to UV radiation?

Answer 12

B. subtilis because it produces endospores

Question 13

The short wavelength UV is able to penetrate through the plastic cover of petri dish.

Answer 13

False

Question 14

Explain the mechanism of action of UV radiation on cells.

Answer 14

UV causes damage to DNA by causing formation of pyrimidine dimers.

Question 15

Which bacteria do we use for the chemical germicide experiment? Check all that apply.

Answer 15

E. coli
S. aureus
P. aeruginosa

Question 16

Which of the following are disinfectants that we will test? Check all that apply.

Answer 16

Lysol
Bleach

Question 17

Which of the following are antiseptics that we will test? Check all that apply.

Answer 17

Isopropyl alcohol
Hydrogen peroxide

Question 18

Arrange the chemical germicide experiment steps in the correct order.

Answer 18

1 coat beads with bacteria

2 dry beads on filter paper

3 incubate beads in germicides

4 place beads into nutrient broth

Question 19

Match the following antibiotics with their mode of action.

Answer 19

Cell wall synthesis and function, vancomycin

50S ribosome inhibition, chloramphenicol

30S ribosome inhibition, tetracyclines

cell membrane permeability, polymyxins

Folic acid synthesis, trimethoprim

DNA/RNA synthesis, rifampin

Question 20

You have just measured the zone of inhibition for several drugs against Staphylococcus. It just happens that all of them are about 14 mm. What does this mean for each drug?

(Use the ‘Zone diameter interpretive chart’ on page 449 of your lab manual, and the ‘Kirby Bauer antibiotic sensitivityLinks to an external site.’ sheet posted on Canvas as resources).

Answer 20

Chloramphenicol, intermediate

Penicillin, resistant

Polymyxin, sensitive

Question 21

These factors determine the size of a zone of inhibition. Mark all that apply.

Answer 21

the thickness of the agar medium

Rate of diffusion

Drug solubility

Question 22

Choose one antibiotic that you would expect to show different effects on Gram-positive and Gram-negative bacteria. Which bacterium is more susceptible? Why do you think this might be the case?

Answer 22

Polymyxin B, Gram positive should be more susceptible since it does not have an outer membrane making it easier for membrane disruption to occur

Question 23

Explain the difference between facultative anaerobes and aerotolerant microbes. Which group has more extensive bioenergetic enzyme system? Explain.

Answer 23

Both can use oxygen, but facultative anaerobes much prefer to use oxygen and are able to produce more energy when they do have access to oxygen.

Facultative anaerobes since they can work with both the present and absence of oxygen.

Question 24

What are the purposes of the indicator strip and the gas generator in the GasPak system in the anaerobic jar?

Answer 24

creates an anaerobic chamber for organisms, the strip is used to indicate the oxygen presence

Question 25

Suppose that the methylene blue strip is blue after incubation, what would you guess the internal environment to be - aerobic or anaerobic?

Answer 25

Aerobic

Question 26

An alternative to the anaerobic jar is a candle jar, in which a candle is placed in the jar, lit, and the lid closed to enable the flame to use the available oxygen. Typically, in this system, not all of the oxygen is used. Which types of organisms would most likely benefit from this environment?

Answer 26

Microaerophiles

Question 27

Undiluted samples are always expressed as 1 while calculating dilutions.

Answer 27

True

Question 28

You want to produce a 10-1 dilution of a bacterial sample using the entire volume. Match the following starting bacterial volume to the amount of water you would add to the tube.

Answer 28

2ml, 18ml

3ml, 27ml

4ml, 36ml

Question 29

You want to produce a 10-2 dilution of a bacterial sample using the entire volume. Match the following starting bacterial volume to the correct amount of water you would add to the tube.

Answer 29

2ml 198ml

3ml 297ml

4ml 396ml

Question 30

Match the following ingredients of EMB to their specific functions.

Answer 30

Nutrients, pancreatic digest of gelatin

differential substrate, lactose

pH indicators, eosin Y and methylene blue

Inhibitors, eosin Y and methylene blue

Question 31

Match the following bacteria with their color on EMB plate.

Answer 31

E. coli green metallic sheen

Coliform pink to purple

Non Coliform colorless

Question 32

Match the following ingredients of HE agar to their specific function.

Answer 32

Nutrients, yeast extract and peptic digest of animal tissue
differentiating substrates, lactose, salicin

Inhibitors, bile salts

pH indicators, bromothymol blue and acid fuchsin

Question 33

Match the following bacteria with the color they show on HE agar.

Answer 33

Salmonella black

Shigella green

Coliform orange

Question 34

Several bacterial samples were inoculated onto Eosin Methylene Blue Agar (EMB) and Hectoen Enteric Agar (HE). Based on the properties described below, select the most likely bacteria species.

Answer 34

This species forms yellow / orange colonies on HE, and pink / purple colonies on EMB., K. pneumoniae

This species forms blue / green colonies on HE, and colorless colonies on EMB. S. sonnei

This species does not grow on either HE or EMB. S. aureus

This species forms orange / pink salmon colonies on HE, and metallic green sheen colonies on EMB. E. coli

This species forms black colonies on HE, and colorless colonies on EMB. S. enterica