lab quiz 2 Flashcards
Match the following reagents with the purpose they serve in Gram stain:
Crystal violet primary stain
Iodine mordant
Alcohol decolorizer
Safranin counterstain
Which of the following statements is true regarding Gram+ bacteria? Check all that apply.
cell wall has a thick peptidoglycan layer
teichoic acids are present
lipopolysaccharide is absent
cell envelope lipid content is relatively low
Which step in the Gram stain is the most critical?
decolorization
In Gram staining, the alcohol acts on:
membrane lipid
What would most likely occur if you applied alcohol for only 1 second?
Gram-negative bacteria would not decolorize like it should, and all bacteria would therefore remain purple (Gram-positive)
+
What would most likely occur if you drenched the bacterial smear in alcohol for 1 minute?
All purple stain would escape as the cell wall of both Gram-positive and Gram-negative bacteria would dissolve
How long does it take to perform and analyze the Gram stain?
A few minutes
You have just performed Gram stain, and forgot to add the iodine. What colors would your Gram-positive (G+) and your Gram-negative (G-) bacteria look like?
G+ pink
G- pink
You have just performed Gram stain, and forgot to do the alcohol decolorization step. What colors would your Gram-positive (G+) and your Gram-negative (G-) bacteria look like?
G+ purple
G- purple
You have just performed Gram stain, and forgot to add safranin. What colors would your Gram-positive (G+) and your Gram-negative (G-) bacteria look like?
G- purple
G+ colorless
You have just performed Gram stain, and realized that you have reversed the order between crystal violet and safranin (safranin-iodine-alcohol-crystal violet). What colors would your Gram-positive (G+) and your Gram-negative (G-) bacteria look like?
G+ purple
G- purple
Match the following bacteria with their shape and arrangement.
Staphylococcus aureus Gram+ cocci
Bacillus subtilis Gram+ rod
Serratia marcescens Gram- rod
Chromobacterium violaceum Gram- rod
Match the following dyes with their purpose for this week’s staining:
Calbofuchsin primary stain for acid-fast
methylene blue counter stain for acid-fast
malachite green primary stain for endospore staining
Safranin counter stain for endospore staining
Arrange the steps of acid-fast smear preparation in the correct order.
1 smear S. aureus in a drop of water
2 air dry and heat fix
3 Chop M. smegmatis on top of S. aureus
4 heat fix and leave slide on warmer until ready to stain
The reason you need to use steam heating for acid-fast staining is because:
High mycolic acid (wax) content in the cell wall of Mycobacterium
++