Lab Quiz #2 Flashcards
Aseptic technique
the method of handling microbes and materials in a way that minimizes contamination
inoculation
process of transferring a microbe from one medium to another
Inoculum
sample being transferred during inoculation
inoculating loop/needle
tools used to transfer bacteria from a culture to sterile media.
isolation streak plate
wide method to separate individual bacteria from mixtures
The goal of a streak plate is ?
to thin out the number of bacteria on a plate to get isolated colonies
Spread-plate method
sample is pipetted onto the plate; spread evenly with a glass spreader
Pour-Plate method
sample is pipetted into the plate and then sterile is poured into the membrane
Agar
provides a solid surface to grow bacteria; from seaweed; Koch developed it
Incubator-
holds a constant temperature, used to grow bacterial cultures
Incinerator-
device used to sterilize inoculating tools
Epidemiology
The study of how a specific infectious agent survives and spreads through a community
Disease Transmission
The actual way the infectious agent spreads
Direct transmission
Biological vectors; or hand holding, shaking and kissing
Portal of Entry
Entranceway through which a specific microbe is able to invade a host
some portals of entry:
- Gastrointestinal tract
- Respiratory tract
- Conjunctiva
- Skin Urogenital tract (STD) —Pregnancy and birth
Reservoir of infection
Any object, living or nonliving, that allows a microbe to grow, reproduce, and maintain its ability to remain infective
fomites
a nonliving object capable of allowing a microbe to survive but not reproduce
what controls fomites?
Proper and consistent hand washing controls fomite transmission
