Culture based Methods
- standardized
- relatively cheap
- easy
Diversity of Assemblage
Species Richness
- number of species
Abundance of each species
Working with microbes
If its open to the air or especially if it has been touches, its contaminated.
Do not:
- touch the sterile swab
- touch the inside of the Petri dish
Act quickly:
- do not leave the Petri dish open any longer than absolutely necessary
Hypothesis
combination of predictions + the reason
Colony Type:
4 traits
- Color
- Form
- Elevation
- Margin
Binary Fission
asexual reproduction through cell division.
Prokaryotes are:
Structurally simple/similar but chemically diverse
Archaea and Bacteria
Cyanobacteria
common prokayote
Identifying Prokaryotes: 4 techniques
Increasing in specificity
- Individual shapes and sizes
- Gram Stain
- Culture
- Molecular methods
Coccus
round or spherical
Bacillus
Rod-shaped
Spirillum
spiral shaped
Gram Stain
differences in peptidoglycan content of cell walls dictate Gram-negative(often pathogenic) and Gram-positive cells.
Culture
using colony traits and selective media to identify species
Molecular
DNA sequencing
