Lab - Prokaryotic Diversity

Question 1

Culture based Methods

Answer 1

• standardized
• relatively cheap
• easy

Question 2

Diversity of Assemblage

Answer 2

Species Richness
- number of species

Abundance of each species

Question 3

Working with microbes

Answer 3

If its open to the air or especially if it has been touches, its contaminated.

Question 4

Do not:

Answer 4

• touch the sterile swab
• touch the inside of the Petri dish

Act quickly:
- do not leave the Petri dish open any longer than absolutely necessary

Question 5

Hypothesis

Answer 5

combination of predictions + the reason

Question 6

Colony Type:

Answer 6

4 traits

1. Color
2. Form
3. Elevation
4. Margin

Question 7

Binary Fission

Answer 7

asexual reproduction through cell division.

Question 8

Prokaryotes are:

Answer 8

Structurally simple/similar but chemically diverse

Archaea and Bacteria

Question 9

Cyanobacteria

Answer 9

common prokayote

Question 10

Identifying Prokaryotes: 4 techniques

Answer 10

Increasing in specificity

1. Individual shapes and sizes
2. Gram Stain
3. Culture
4. Molecular methods

Question 11

Coccus

Answer 11

round or spherical

Question 12

Bacillus

Answer 12

Rod-shaped

Question 13

Spirillum

Answer 13

spiral shaped

Question 14

Gram Stain

Answer 14

differences in peptidoglycan content of cell walls dictate Gram-negative(often pathogenic) and Gram-positive cells.

Question 15

Culture

Answer 15

using colony traits and selective media to identify species

Question 16

Molecular

Answer 16

DNA sequencing