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Microbiology II Lab > Lab Practical 3 > Flashcards

Lab Practical 3 Flashcards

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Biology 101 flashcards
1
Q

Spore forming G+ bacilli

A

Bacillus
Clostridium, clostridioides (anaerobes)

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2
Q

Catalase positive G+ bacilli

A

Bacillus, corynebacterium, listeria

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3
Q

Acid fast stain mechanism

A

Ziehl neelsen - hot, heat makes dye penetrate cell wall
Kinyoun - cold, phenol dissolves lipid material and penetrates

Method: carbolfuchin stain, then counterstain

Appearance: bacteria red, other stuff blue

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4
Q

Modified acid fast stain for who? How does it work?

A

Aerobic actinomycetes
- Weak acid decolorizer instead of 3% HCL for mycobacteria

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5
Q

Which G+ rod grows on SBA and CHOC?

A

All Bacillus, Listeria, most corynebacterium but some don’t grow on CHOC like jeikeum
Nocardia

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6
Q

Corynebacterium diphtheriae special media is called and list properties

A

Cystine tellurite agar/modified tinsdale agar
- tellurite inhibits other growth and can reduce cystine
- positive for diphtheria: gray-black colonies

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7
Q

What special nutritional requirments do anaerobes have

A

Vitamin K and hemin

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8
Q

List the three main anaerobes

A

Clostridium, clostridioides, actinomyces

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9
Q

G+ rods and growth timing

A
  • Bacillus: within 24 hours
  • Listeria, Erysipelothrix, coryneform: within 48 hours
  • Nocardia and aerobic actinomycetes: within 3-6 days and up to 4 weeks
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10
Q

G+ bacilli growth conditions

A
  • Bacillus: 35-37 or in 5% CO2
  • Listeria, erysipelothrix, coryneform: 35-37 air or 5% CO2
  • Nocardia, aerobic actinomycetes: 37 C
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11
Q

Mycobacteria growth requirements

A

Strict aerobes, slow growing
30-45 C

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12
Q

Bacillus anthracis vs other bacillus on SBA

A

B. anthracis is non hemolytic and has filamentous “medusa head” colony growths, non motile

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12
Q

Elek test for what and how does it work

A

Immunodiffusion, tests for toxin of diphtheria

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13
Q

Listeria monocytogenes key characteristics

Hemolysis, motility, bile esculin, camp

A

Hemolysis: narrow beta
Motility: umbrella or tumbling at 25 C
Bile esculin: +
CAMP: +

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14
Q

Specimen collection for anaerobes

A
  • Abscesses
  • Aspirates
  • Fluids
  • Tissue

Swabs are discouraged

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15
Q

Gram stain of Clostridium and clostridioides

A

G+, spore forming rods
C. perfringens can be box car shaped

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16
Q

Gram stain of Actinomyces

A

G+, non spire forming, branching bacilli

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17
Q

Medium for bacteroides fragilis

A

BBE agar
Bacteroides bile esculin

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18
Q

Medium for bacteroides and prevotella

A

KVLB agar
Kanamycin-vancomycin laked blood agar

19
Q

Medium for anaerobic bacteria

A

PEA agar
Phenylethyl alcohol agar

20
Q

Clostridioides difficile medium

A

Cycloserine cefoxitin fructose CCFA agar

21
Q

Gram stain for corynebacterium

A

V or L shape,clubs, palisades, G+ pleomorphic

22
Q

List the 4 Misc. gram neg rods

A

Acinetobacter baumanni
Burkholderia cepacia
Stenotrophomonas maltophilia
Pseudomonas aeruginosa

23
Q

Misc G- rods that are oxidase +

A

Burkholderia cepacia and pseudomonas aeruginosa

24
Q

Only misc G- rod that is non motile

A

Acinetobacter baumanni

25
Q

Campylobacter special medium

A

Campylobacter blood agar CAMPY-BA. selective enriched for C. jejuni from stool

26
Q

Special medium for Vibrio

A

TCBS
Thiosulfate citrate bile salt sucrose agar

27
Q

O/F medium mechanism

A

Two tubes, one aerobic and one anaerobic
- turns fully yellow if fermenter
- turns slightly yellow if oxidizer
- oil layer for true anaerobic conditions

28
Q

Pigment production on MH medium

A

Pseudomonas aeuroginosa

29
Q

Vibrio special growth requirements

A

Increased sale for all EXCEPT for V. cholerae

30
Q

Who has darting motility

A

Campylobacter

31
Q

O/129 disk susceptibility

A

Vibrio is sus

32
Q

Campylobacter special gram stain appearance

A

S shape, sea gull wings

33
Q

Burkholderia mallei vs pseudomallaei test differentiation

A

Mallei oxidase: -, variable, non motile

Pseudomallei oxidase: +, motile

34
Q

Key points for lower respir tract diagnosis and collection

A

First morning sputum is the best

35
Q

Key points for diagnosis upper respir. tract

A
  • swabs are NOT for otitis media or sinitus
  • Aspirates are better
  • Throat specimens use thorough swabs
36
Q

CNS diagnostic collection

A
  • Before antimicrobial therapy
  • Do not fridge CSF
  • 2-4 blood cultures if bacterial meninigitis suspected
37
Q

Clostridium difficile stool collection requirement

A

DIARRHEA WET SLOPPY LOOSE STOOL ONLY

38
Q

UTI diagnostic collection key points

A
  • If 3+ bacteria, suspect contamination
39
Q

When do you need to work up a colony on a stool MAC

A

Clear colorless, rule out shigella and salmonella

40
Q

When do you need to work up a colony on stool SMAC

A

Clear colorless, rule out O157

41
Q

When do you need to work up a colony on stool HE

A

Blue/blue green (w or w/o black)
Rule out salmonella and shigella

42
Q

Urine specimen colony count

A

1 microliter loop: multiply by x1000
10 microliter loop: multiply by x100

43
Q

Direct smear report numbers

A

10x objective
WBCs
- 4+, >25
- 3+, 11-25
- 2+, 3-10
- 1+, <3

SEC
- >10 or <=10

Bacteria under oil
- 4+, >10
- Report gram and morph

44
Q

Oxacillin/methicillin resistant staphylococci

what about it, what to do next

A
  • Possess mecA gene
  • Report as resistant to all beta-lactams
  • Test with cefoxitin to predict mecA better

Microbiology II Lab (4 decks)

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