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Microbiology II Lab > Lab Practical 2 > Flashcards

Lab Practical 2 Flashcards

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Biology 101 flashcards
1
Q

All GN Cocci

key characteristic

A

Neisseria gonorrhoeae
Neisseria meningitidis
Neisseria lactamica
Moraxella catarrhalis

kidney bean shaped

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2
Q

All GN dipplococci and fastidious GN bacilli

A

Haemophilus influenzae
Haemophilus parainfluenzae
Brucella
Francisella tularensis
Legionella

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3
Q

All Enterobacterales

A

Citrobacter freundii
Enterbacter cloacae
Escherichia coli
Klebsiella pnemoniae
Proteus mirabilus
Vibrio
Shigella
Salmonella
E. coli O157

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4
Q

All Remaining MISC GNR

A

Acinetobacter baumannii (ACBA)
Burkholderia cepacia (BUCE)
Stenotrophomonas maltophilia (STMA)
Pseudomonas aeruginosa (PSAE)
Yersinia

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5
Q

MAC Agar

type, additives, growth indicators

A

Slective differential
Selective: bile salt + crystal violet, selects for G-
Differential: lactose fermentation > turns agar pink
Growth: enterobacterales mostly

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6
Q

All Mediums for Neisseria

3 type, reagents

A

Thayer Martin, Modified Thayer Martin, Martin Lewis

All are modified CHOC with vancomycin

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7
Q

What Agar is Used to Isolate Bordatella?

2 name, type, reagents

A

Bordet-gengou (B-G) blood agar
- Enriched + selective
- Blood and glycerol for enrichment, antimicrobials for selectivity
- For B. pertussis and parapertussis

Regan-Lowe agar
- B. pertussis
- Charcoal

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8
Q

Legionella Isolating Medium

Name, type, how

A

BCYE-alpha (buffered charcoal-yeast extract) Agar
- Enriched
- L-cysteine added which Legionella needs
- Charcoal absorbes toxic compounds

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9
Q

SMAC Agar

type, how, what for

A

Selective differential
- Sorbitol replaces lactose
- Normal E. coli flora can ferment sorbitol, E coli. O157 is colorless

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10
Q

HE Agar

what species, reagents,

A

Salmonella and Shigella
- Lactose, sucrose, bile salt, ferric ammonium sulfate for H2S
- Salmonella produces H2S, Shigella does not

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11
Q

CIN Agar

2 species, reagent

A

Isolation of Yersinia, sometimes Citrobacter can grow
- Yersinia appears translucent without dark red centers
- Novobiocin antimicrobial

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12
Q

Medium for Vibrio Isolation

A

Thiosulfate Citrate Bile salts Sucrose (TCBS) agar
- Thiosulfate
- oxgall
- sucrose use = yellow color

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13
Q

Neisseria + Moraxella Lactose

A

Neisseria gonorrhoea: -
Neisseria lactamica: +
Moraxella: -
Neisseria meningitidis: -

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14
Q

Neisseria + Moraxella Glucose

A

All Neisserias can use
Moraxella cannot use

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15
Q

Neisseria + Moraxella Maltose

A

Lactamica and meningitidis can
Gonorrhoea and Moraxella cannot

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16
Q

Butyrate test

method and who is +

A

Production of butyrate esterase causes release in indoxyl. Forms indigo, blue green color change
Only Moraxella catarrhalis is +

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17
Q

Oxidase

method

A

Kovac reagent oxidized by electrons, makes purple color

18
Q

Porphyrin

method, what for, results

A

Porphobilinogen presence, ALA will convert to porphyrin, fluorescent

  • Separate H. influenzae and parainfluenzae
  • Tests for hemin

+: parainfluenzae
-: influenzae

19
Q

Satellite Growth

A

H. influenzae needs both NAD (V) and hemin (X) that it will grow around S. aureus bc it lyses the blood and releases V factor

20
Q

Oxidation Fermentation (O/F)

how, what species and results

A

How: sugar and pH indicator, oil layer for anaerobic
Fermenter: E. coli
Oxidizer: Pseudomonas aeruginosa

21
Q

Pigment Production of Pseudomonas Aeruginosa

A

blue and fluorescent coloring

22
Q

Spot Indole

reagents, interpretation

A

Trypyophanase enzyme detection, degrade indole because of degradation of tryptophan
- Red color +

Separate E. coli from other enterbacterales becaue E.coli is +

23
Q

ONPG Test

method, results

A

B- galactosidase cleaves ONPG into galactose
- Positive if broth turns yellow
E.coli is + while Proteus mirabilis is -

24
Q

MUG test

method, results

A

Tests for beta glucuronidase, fluorescence
Only E. coli is +

25
Q

TSI Test

method, results

A

Glucose, sucrose, lactose, ferric and sodium thiosulfate
A/A: glucose + something else
K/A: only glucose
K/K: none of the sugars
H2S: has to be either A/A or K/A because enterbacterales always uses glucose, only ones that can produce H2S

26
Q

Motility Test

A

Nonmotile enterobacterales: Klebsiella pnuemoniae and Shigella
Nonmotile Misc GNR: acinetobacter baumannii

27
Q

Citrate Agar

A

Can utilize citrate, slant turns blue
Klebsiella pneumoniae +
E. colli -

28
Q

Good Test to Differentiate Proteus Mirabilis

But also what?

A

Urea hydrolysis
- CIFR, ENCL, and KLPN and also split urea into ammonia

29
Q

MR/VP

2 reagents in VP, enterobacterales

A

VP: KOH and alpha-naphthol
Positive for VP: ENCL and KLPN
- Everything else negative
- Vice versa for MR: ENCL and KLPN are negative for MR while everything else +

30
Q

Nitrate Reduction

what kinds of reduction? 2 reagents in broth

A

All enterobacterales can reduce nitrate
- Can go directly to N2 gas or
- alpha napthylamine and sulfanilic acid for reduction to nitrite
- Turns red if nitrite
- If colorness, add zinc and red color means there is still nitrate, no color means N2 produced and no nitrate left

31
Q

Yersinia Bench Aid

A
  • Catalase +
  • Nonmotile at 25 and 35/37
  • Urease, Oxidase, Indole -
  • ## Bipolar staining safety pin appearance
32
Q

Burkholderia Bench Aid

A

B. mallei: oxidase -, non motile
B. pseudomallei: opposite of that

33
Q

Francisella Bench Aid

A
  • No SBA growth
  • Oxidase, urease -
  • Catalase weak +
34
Q

Brucella Bench Aid

A

SBA and CHOC
- Oxidase, urease +
- Non motile

35
Q

Oxidase + Rods

2, differentiate

A

Burkholderia cepecia
Pseudomonas aerogunosa
- Then do colony morph, pseudomonas smell and sheen

36
Q

Nonmotile Rods

3, differentiate

A

Klebsiella pneumoniae
Shigella
Acinetobacter baumannii
- MAC lactose, ACBA is non fermenter

37
Q

Motile Rods

5, how to differentiate

A

ESCO
ENCL
STMA
PRMI
CIFR
- Then do tsi h2s production

38
Q

TSI H2S Production in Rods

A

+ : PRMI, CIFR
- : ENCL, ESCO, STMA

39
Q

Differentiate ESCO and ENCL

A

Indole
- E. coli is +
- Enterobacter cloacae is -

40
Q

Differentiate CIFR and PRMI

A

Proteus mirabilis swarms on SBA, Citrobacter does not

Microbiology II Lab (4 decks)

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