Lab Practical Flashcards
Streptococcus pneumonia
-Alpha Hemolytic
-Sensitive to “P”- optochin
-Gram-(+) w/ a capsule
Escherichia coli
-Gram-(-)
-Beta-hemolytic
-Indole (+)
-Ferments lactose
Staphylococcus aureus
-gram-(+) cocci
-H2O2 (+) (catalase positive)
-Clots rabbit plasma (coagulase (+))
Staphylococcus epidermidis
-Gram-(+) cocci
-H2O2 (+) (catalase positive)
-gamma-hemolytic
-coagulase (-)
Micrococcus luteus
-gram-(+) cocci
-H2O2 (+) (catalase positive)
-gamma-hemolytic
-coagulase (-)
Proteus mirabilis
-Urea (+) STRONG
-swarms
Streptococcus agalactiae
-Beta-hemolytic
-catalase (-)
-Gram-(+) cocci
Streptococcus pyogenes
-Beta-hemolytic
-Catalase (-)
-Gram-(+) cocci
-sensitive to bacitracin (“A”)
Enterococcus faecalis
-Gram-(+)
-catalase (-)
-Gamma-hemolytic
Streptococcus viridans
-Alpha-hemolytic
-catalase (-)
-Resistant to “P”- optochin
Pseudomonas aeruginosa
-Weak urea (+)
-Gram-(-)
-beta-hemolyic
Treponema pallidum
Spirochete
Eosin Methylene Blue agar
-differential for lactose fermentation
-E. coli, P. aeruginosa, Proteus mirabillis grow on this media
-selective (eosin and methylene blue)
-used to isolate and differentiate gram-(-) bacteria
Chocolate agar
Neisseria and Haemophilus grow on this media
-nutritive and enchriched (w/ 5% sheep’s blood) media
-both gram-(+) and gram-(-) grow on this media
MacConkey agar
-differential lactose fermentation
-E. coli, P. aeruginosa, and Proteus mirabillis grow on this media (gram-(-) bacilli)
-Media type: selective and differential
-bile salts and crystal violet make it selective
-lactose makes it differential
-differentiate lactose fermenters (pink) from Non lactose fermenters (plate yellow)
-used to isolate and differentiate gram-(-) bacteria
Mannitol Salt Agar
-different for mannitol fermentation
-S. aureus, S. epidermidis grow on this media (Staphylococci)
-Media type: selective and differential
-7.5% salt makes it selective
-mannitol makes it differential
-differentiates S. aureus from other Staphylococcus species
-and isolate salt tolerant bacteria
Colistin Nalidixic Agar
-Staphylococcus, Micrococcus, Streptococcus, and Bacillus grow on this media (GRAM-(+))
-Media type: Selective
-colistin and nalidixic acid make it selective
-isolates gram-(+) bacterial cause
Trypticase Soy Agar
-micrococcus, S. aureus, Bacillus subtilis, Streptococci, Staphylococci, Pseudomonas, E. coli grow on this media
-media type: nutritive
-grows both gram (+) and (-)
Trypticase Soy Broth
Micrococcus luteus, Escherichia coli, Proteus mirabilis, Streptococci, Staphylococci, Pseudomonas aeruginosa, Bacillus, Candida albicans grow on this media
-media type: nutritive
-both gram (-) and (+) grow on this media type
Bile Esculin Agar
-Enterococcus grows on this media
-differential for esculin hydrolysis
-media type: selective and differential
-selective (bile salts)
-differential (lactose)
Blood agar
-S. pyogenes, S. agalactiae, S. pneumoniae, S. viridans only grow on this media (Fastidious streptococci)
-differential for hemolysis
-Media type: Enchriched and differential and nutritive
-Sheep RBC’s make it differential
-differentiates hemolytic strepts
-grows both gram (+) and (-)
Sabouraud dextrose agar (SDA)
-media type: selective
-TSA w/ reduced 5.6pH
-used to isolate yeast
-Candida albicans grows on this agar
