Lab Practical Flashcards

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1
Q

Independent variable

A

variable that is the experimental condition

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2
Q

dependent variable

A

variable that is being measured

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3
Q

g —- > mg

A

1g = 1000mg

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4
Q

mL —- microliter

A

1mL = 1000microliters

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5
Q

fast plants (dom, recessive)

A
  • dominant: Purple stem, green leaf
  • recessive: Green stem, green-yellow leaves
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6
Q

Anthocyanins

A

pigments that cause purple stem

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7
Q

Expected dist of fast plants

A

9:3:3:1 (purple stem and green leaf to green stem and yellow-green leaves)

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8
Q

What’s used to measure pH

A

red cabbage (anthocyancins)

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9
Q

pH color scale

A

acidity: red
alkalinity: blue/green
neutral: purple

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10
Q

white grape juice pH

A

1

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11
Q

7up pH

A

4

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12
Q

white wine pH

A

3

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13
Q

seltzer water pH

A

6.5

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14
Q

milk of magnesia (mg(oh)2)

A

10

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15
Q

sodium bicarbonate

A

9

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16
Q

maalox

A

7

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17
Q

pine sol

A

2

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18
Q

mouthwash

A

8

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19
Q

shower cleanser

A

8

20
Q

pH of a buffer

A

6-8

21
Q

Benedict’s Test

A

test for monosaccrides (glucose)
- positive: orange
- negative: blue

22
Q

Juice for Benedict’s test?

A

onion juice has more reducing sugars than potato

23
Q

Iodine Test

A

tests for starch
- pos: purple
-neg: yellow

24
Q

Juice for iodine Test

A

Potato juice has more starch

25
Q

Biuret test

A

tests for peptide bonds
- pos: purple
-neg: yellow

26
Q

biuret test solubility in oil

A

-acetone dissolves
-water does not
experiment tested salad dressing

27
Q

blepharisma

A

look pic

28
Q

scenedesmus

A

look pic

29
Q

volvox

A

look pic

30
Q

Dialysis Sac

A
  • iodine in beaker (yellow, dark)
  • dialysis sac with iodine ( clear, dark)
  • iodine diffused inside sac and glucose went out
31
Q

elodea

A

dehydrated with NaCl
- chloroplasts all over
- shrunk appearance

32
Q

Potato

A
  • gained weight h2O
  • lost weight dehydrated
33
Q

why do leaves turn dark

A

indication of starch

34
Q

chromatography does what

A

plant pigments

35
Q

chromatography

A

look pic

36
Q

plant pigments seperated based on…

A

polarity (paper=polar)

37
Q

idenitfy mitosis stages

A

look pic

38
Q

genotype

A

geneitc makeup

39
Q

phenotype

A

physically visible characterustuics

40
Q

taster

A

GG/CC
-has restrcition enzyme on site

41
Q

non-tatser

A

GGCC
- no restriction enzyme on site

42
Q

super taster

A

GG/CC
- restrcition ensyme on site

43
Q

LB

A

(-): all over gorwth
(+): all over growth
lysogeny broth nutritional broth

44
Q

LB/AMP

A

(-): no growth
(+): all over growth
amplicillin (antibiotic)

45
Q

LB/AMP/pARA-R

A

(-): no growth
(+): growth (pinK)
- has plasmid pARA-R added via CaCL
(arabinose operon. carbon and food source)