Lab Practical Flashcards
Independent variable
variable that is the experimental condition
dependent variable
variable that is being measured
g —- > mg
1g = 1000mg
mL —- microliter
1mL = 1000microliters
fast plants (dom, recessive)
- dominant: Purple stem, green leaf
- recessive: Green stem, green-yellow leaves
Anthocyanins
pigments that cause purple stem
Expected dist of fast plants
9:3:3:1 (purple stem and green leaf to green stem and yellow-green leaves)
What’s used to measure pH
red cabbage (anthocyancins)
pH color scale
acidity: red
alkalinity: blue/green
neutral: purple
white grape juice pH
1
7up pH
4
white wine pH
3
seltzer water pH
6.5
milk of magnesia (mg(oh)2)
10
sodium bicarbonate
9
maalox
7
pine sol
2
mouthwash
8
shower cleanser
8
pH of a buffer
6-8
Benedict’s Test
test for monosaccrides (glucose)
- positive: orange
- negative: blue
Juice for Benedict’s test?
onion juice has more reducing sugars than potato
Iodine Test
tests for starch
- pos: purple
-neg: yellow
Juice for iodine Test
Potato juice has more starch
Biuret test
tests for peptide bonds
- pos: purple
-neg: yellow
biuret test solubility in oil
-acetone dissolves
-water does not
experiment tested salad dressing
blepharisma
look pic
scenedesmus
look pic
volvox
look pic
Dialysis Sac
- iodine in beaker (yellow, dark)
- dialysis sac with iodine ( clear, dark)
- iodine diffused inside sac and glucose went out
elodea
dehydrated with NaCl
- chloroplasts all over
- shrunk appearance
Potato
- gained weight h2O
- lost weight dehydrated
why do leaves turn dark
indication of starch
chromatography does what
plant pigments
chromatography
look pic
plant pigments seperated based on…
polarity (paper=polar)
idenitfy mitosis stages
look pic
genotype
geneitc makeup
phenotype
physically visible characterustuics
taster
GG/CC
-has restrcition enzyme on site
non-tatser
GGCC
- no restriction enzyme on site
super taster
GG/CC
- restrcition ensyme on site
LB
(-): all over gorwth
(+): all over growth
lysogeny broth nutritional broth
LB/AMP
(-): no growth
(+): all over growth
amplicillin (antibiotic)
LB/AMP/pARA-R
(-): no growth
(+): growth (pinK)
- has plasmid pARA-R added via CaCL
(arabinose operon. carbon and food source)