Lab Midterm Flashcards
What is plasma minus clotting factors and fibrin?
serum
What is blood minus cells?
plasma
What are the 3 essential requirements for having an optimal precipitation rxn?
Ab is bivalent
Ag is multvalent
ratio of Ag to Ab in equivalence zone
What is the zone of Ag/Ag rxn where one Ag molecule is bound by 2 Ab molecules with no cross linking?
antibody excess zone
Which zone would be seen in an Ag/Ab precipitation reaction for diagnosing immune complex dz?
antigen excess zone
What is the term for an agar double diffusion of 2 organism extracts that share common antigen epitopes andd therfore a common line of preciptin?
line of identity
What is the term for agar double diffusion that antigens only share soe antigenic determinants?
spur (lines of partial identity)
What is seen in vivo with a antibody excess zone in a precipitation reaction?
Ab present, but not enough Ag, macrophages dispose of complexes
What is seen in vivo in the equivalence zone?
Ag = Ab, insoluble complexes are easily disposed of
What happens in vivo with an antigen excess zone?
immune complexes form because of antigen excess, they are soluble, DISEASE
Term for strength of overall multivalent binding
avidity
Term for combined strength of bonding b/w Fab and epitope on Ag
Affinity
What is agglutination?
rxn of PARTICULATE Ag with Ab
What types of Ab can cause hemagglutination?
Bivalent (IgG) or multivalent (IgM
Term for outgrowths of the proliferating synovia in RA?
pannus
