Lab Midterm—1 Flashcards
What is denaturation?
protein loosing shape in high temp or low pH
What enzyme is made in the stomach?
pH?
Pepsin
~ 2
What enzymes are made in the SI?
pH?
Trypsin, chymotrypsin, carboxypeptidase
~ 9
What does the biuret test monitor for?
presence of protein
What does the ninhydrin test monitor for?
amino acids
When all of the protein has been hydrolyzed, which test should be test positive? negative?
positive: ninhydrin
negative: biuret
The biuret turns from blue to violet when positive for proteins. T or F?
T
The ninhydrin test turns from colorless to what color when positive for amino acids?
blue-violet
What must first take place for fats to be digested efficiently?
emulsification
what breaks down lipids during emulsification?
bile salts
chylomicrons are absorbed by which cells?
lacteals (lymph vessels)
What does a positive control include when analyzing lipid digestion?
substrate, functional enzyme, bile salts
What does a negative control include when analyzing lipid digestion?
missing a substrate, functional enzyme, or bile salt
What 2 tests monitor the progress of lipid digestion progress?
iodine
Benedict test
A positive iodine test is what color?
blue-black
