Lab Glassware Flashcards
used for heating and sterilization, commonly
used for constriction of reagent bottles
High Thermal Resistant/Borosilicate Glass
high resistance to
alkali
Boron-free/Soft Glass/ high alkali resistant glass
special alumina-silicate glass that has
been strengthened chemically than thermally
Corex/high silica glass
poor resistance to high
temperature; used to make disposable
glassware
Flint glass/soda lime glass
used for light sensitive
reagents
Low actinic glass
Are frequently used
for preparation of standard solutions
Volumetric flask
Wide straight sided cylindrical
vessels that are available in many sizes;
used generally for mixing and for
reagent preparation
Beaker
Are often used for
preparing reagents and titration
purposes
Erlenmeyer flask
Test tubes comes in
different sizes depending on their
intended use; chemical reaction
medium
Test tube
A long and straight
sided cylindrical piece of glassware with
calibration
Graduated cylinder
Long cylindrical graduated
pipettes with stopcock (glass or Rubber);
used for titration
Biuret
delivers the exact amount it
holds into a container
Calibration mark To deliver
holds the particular volume
but does not dispense the exact volume
Calibration mark To contain
etched rings on top of the pipette;
exact volume is obtained when the last drop
is blown out
Blowout pipette
absence of etched ring; liquid
is allowed to drain by gravity
Self- draining pipette
Type of Transfer Pipette
Volumetric pipette
Pasteur pipette
Automatic pipette
for
nonviscous fluid; self draining
Volumetric pipette
transfers fluid
w/o consideration of a specific
volume
Pasteur pipette
Types of graduated pipette
Serologic pipette
Mohr pipette
w/
graduation to the tip; blowout
Serologic pipette
w/o graduation
to the tip
Mohr pipette
An instrument that uses centrifugal
force to separate solid matter from a
liquid suspension
Centrifuge
Handling Solutions
- When pouring contents from a
reagent bottle into an open-mouth
container such as a beaker, use a
stirring rod. - Remove the glass stopper from
reagent bottles by pulling it with the
use of index and middle fingers,
palm facing upward
Using the Laboratory Pipette: If a _________ portion of the liquid is to be
transferred to another container, use a
pipette or a medicine dropper
small portion
Using the Laboratory Pipette: Let the pipette be filled by means of_________ action
capillary action
Using the Laboratory Pipette: To hold the liquid in, place the ______ finger at
the open end of the pipette
index finger
Using the Laboratory Pipette: If exact amounts of solutions are needed, use
an _________.
aspirator
Heating solutions in a
Test tube:
Fill use make and place
Heating solutions in a Test tube: Fill the test tube with ________ of liquid to be
heated
half full
Heating solutions in a Test tube: Use a test ______ holder to hold the test tube
tube holder
Heating solutions in a Test tube: Make sure the test tube is positioned ______ degrees
angle when heating it
45 degrees
Heating solutions in a Test tube: Place it over the flame passing it back and forth
so that the flame strike the tube just below at the liquid level but never at the __________ of the tube
bottom tube
What not to do Heating solutions in a Test tube
- Never point the open end of the tube to anyone while heating the
solution in it - Never look directly inside the tube that is being heated
- Never smell the solution directly from the test tube, wave your hand
towards you to identify the smell
Handling Solids
Spoon
Put
Donot place
Donot return
Handling Solids: To get solids from a reagent bottle, spoon it with a clean and dry ________ (a dirty spatula will contaminate the portion being taken and all the reagents inside the bottle)
clean and dry spatula
Handling Solids: When weighing solid chemicals, put the reagent on a ______ glass
watch glass
Handling Solids: Do not place the reagent directly on the pans of the _______
balance
Handling Solids: Do not return excess reagents into its __________ container
original container
it is the process
of separating solid particles
from liquid by pouring the
mixture in a filter paper
Filtration
Filtration it is the process
of separating solid particles
from liquid by pouring the
mixture in a __________
filter paper
solid left on the
filter paper
Residue
the liquid portion at
the bottom of the container
Filtrate
It is the process of pouring the liquid
portion of the mixture to another container
while not disturbing the solid particles that
settle at the bottom of the container
Decantation
solid at the bottom of the
container
Residue/ sediment
the liquid portion on top of the
solution
Decantate
