[Lab] Flagellates Part 2 Flashcards
Does the Trichomonas spp. have a cyst stage
No
True or false: Trichomonas spp. has a rounded anterior end and somewhat pointed
posterior end.
True
True or false: Trichomonas spp. has a straight and soft axostyle
False (curved and rigid)
True or false: Trichomonas spp. has a small cytostome on one side of the
anterior end.
True
Shape of nuclei of Trichomonas spp.
Spherical
Trichomonas spp. has a blepharoplast
Yes
Blepharoplast is present margin from which 3-5
flagella and a marginal flagellum on an undulating membrane, the base attachment being a heavy costa.
Size of T. vaginalis trophozoite
15-20
Shape of T. vaginalis trophozoite
Pyriform
True or false: the axostyle of T. vaginalis trophozoite is prominent
True
Number of flagella of T. vaginalis trophozoite
4 (all are anterior)
What will you see in the cytoplasm of T. vaginalis trophozoite
Siderophil granules
Motility of T. vaginalis trophozoite
Jerky (tumbling), fast
Where does the T. vaginalis live in a male host
Epithelium of the urethra, prostate gland
Where does the T. vaginalis live in a female host
Surface of vaginal epithelium
True or false: Male hosts of T. vaginalis are always symptomatic
False (usually asymptomatic carriers)
MOT of T. vaginalis
- Sexual intercourse
- Contaminated toilet seat and towel
True or false: T. vaginalis is the only pathogenic Trichomonas
True
Disease caused by T. vaginalis
Trichomoniasis
Clinical manifestations of T. vaginalis
- Inflamed vaginal walls with petechial hemorrhage.
- Surface of the vaginal epithelium is covered with coagulated materials: leukocytes, RBC and Trichomonas
- Desquamation of the epithelial cells
- Cause non-specific urethritis, vaginitis and prostatitis.
Signs and symptoms of Trichomoniasis
- Greenish yellow frothy vaginal
discharge - Intense itchiness of the vagina
- Burning sensation
- Strawberry cervix
Technique/s used for the recovery of T. vaginalis
- Unstained saline wet preparation (TROPHOZOITE)
Specimen of interest for T. vaginalis (male)
a. Semen
b. Prostatic secretions
Specimen of interest for T. vaginalis (female)
a. Urine
b. Urethral discharges
c. Vaginal discharges
d. Cervical secretion
Stains used for recovery of T. vaginalis
- Giemsa
- Pap’s
- Romanowsky
- Acridine Orange
Culture media for T. vaginalis
a. Diamond’s modified medium
b. Feinberg and Whittington culture media
c. Cysteine Peptone Liver Maltose
d. Simplified Trypticase Serum
Treatment for T. vaginalis
- Oral metronidazole (both sexes)
- Acid douche (females)
Prevention and control for T. vaginalis
- Proper personal hygiene .
- Avoid promiscuous sexual intercourse.
- Protective devices such as condoms should be used.
Size of T. tenax trophozoite
5-12
No. of flagella of T. tenax trophozoite
4
Does T. tenax have an undulating membrane
Yes
Does T. tenax lack a free posterior extension
Yes
T. tenax lives in
Oral cavity
MOT of T. tenax
Droplet spray from the mouth (Kissing,
common use of contaminated dishes and drinking glasses)
Lab diagnosis of T. tenax
- Swabbing the tartar between gingival margin of the
gums or the tonsillar crypts. - Direct examination of mouth scrapings.
Size of T. hominis trophozoite
7-13
No. of flagella of T. hominis trophozoite
5 (anterior)
Where does T. hominis live
Colon
MOT of T. hominis
Ingestion of contaminated food and water