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Bac-T > Lab Final > Flashcards

Lab Final Flashcards

1
Q

Which type of fungi has lots of macroconidia?

A

Microsporum

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2
Q

Which type of fungi has lots of microconidia?

A

Trichophyton

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3
Q

When do you use TSA agar?

A

General purpose

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4
Q

When do you use blood agar?

A

Fastidious organisms (need enriched media)

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5
Q

What diagnostic characteristic does blood agar let us utilize?

A

Hemolysis

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6
Q

When type of gram bacteria would you use MacConkey agar for?

A

G –

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7
Q

What diagnostic characteristic does MacConkey agar let us utilize?

A

Lactose fermentation

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8
Q

What aspects of MacConkey agar inhibit G+ bacteria?

A

Bile Salts, Crystal Violet

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9
Q

What aspect of MacConkey agar stain the lactose?

A

neutral red dye

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10
Q

Non lactose fermenters that grow on MacConkey utilize what nutrient?

A

Peptone

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11
Q

Lac- bacteria that grow on MacConkey?

A

Salmonella, Proteus, Pseudomonas, Shigella

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12
Q

Lac+ bacteria that grow on MacConkey? (just the three specifically addressed in lab)

A

E. coli, Enterobacter, Klebsiella

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13
Q

“Primary stain” of Gram Stain process?

A

Crystal Violet

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14
Q

“Mordant stain” of Gram Stain profess?

A

iodine

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15
Q

Decolorizer for gram staining?

A

Alcohol or acetone

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16
Q

“Counterstain” for gram staining?

A

Safranin/Fuschin

17
Q

What does crystal violet stain for gram staining?

A

BOTH G- and G+

18
Q

What does Safranin/Fuschin stain for gram staining?

A

G- ONLY (stains pink)

19
Q

What is catalase used for?

A

Differentiate Staph from Strept

20
Q

Is Staph or Strept Cat+?

A

Staph

21
Q

What does oxidase test test for?

A

Cytochrome C Oxidase

22
Q

What does catalase test for?

A

conversion of H202 into H20 and 02

23
Q

Is pseudomonas or strept Ox+?

A

Pseudomonas

24
Q

What agent is added for the motility test?

A

Tetrozolium salts

25
Q

What are tetrazolium salts converted to by motile bacteria?

A

Formazan (red color)

26
Q

What is the indicator for Urease test?

A

phenol red

27
Q

What color is negative/positive for the urease test?

A

Yellow- Negative

Red- Positive

28
Q

What antimicrobial susceptibility tests allow us to get an MIC?

A

E-test and Broth Microdilution

29
Q

Disk diffusion antimicrobial susceptibility test?

A

Kirby-Bauer

30
Q

Micro-ID test is used to differentiate which group of bacteria?

A

Enterobacteriaceae

31
Q

What McFarland standard is used for antimicrobial susceptibility?

A

0.5

32
Q

What is the colony forming units/mL for the 0.5 McFarland?

A

1.5x10^8

33
Q

Which antimicrobial susceptibility test utilizes Mueller-Hinton?

A

Broth Microdilution

34
Q

Competitive ELISA tests for what?

A

Antigen

35
Q

Indirect ELISA tests for what?

A

Antibody

36
Q

Sandwich ELISA tests for what?

A

Antigen

37
Q

Which IFA test is brighter?

A

Indirect

Bac-T (4 decks)

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