Lab Experiment 6a Flashcards
What are the strong indications of typhoid infection?
A positive tubex result together with clinical symptoms of typhoid fever.
What is Tubex TF?
A 10-minute semi-quantitative, in vitro, diagnostic serum assay, for the detection of typhoid fever caused by Salmonella typhi.
Tubex detects the presence of __________?
Anti-O9 antibodies in the patient’s serum
How does Tubex TF detect anti-O9 antibodies?
By assessing their ability to inhibit the reaction between the antigen coated brown and antibody coated blue reagents
The level of ___________ is proportional to _______ of anti-O9 antibodies in the sample.
Inhibition; Concentration
How is separation enabled in Tubex test?
By magnetic force
How many uL of Tubex TF Brown Reagent is added to each well?
45 uL
How many uL of Tubex TF positive or negative control is to be added in the experiment? And how many times does it need to be pipetted up and down?
45 uL. 10 times
How many Tubex TF Blue Reagent is added to each well?
90 uL
How many minutes do we shake the Tubex Reaction Well Strip?
2 minutes
How long does it take for the separation to obtain a clear supernatant?
5 minutes
Reading should be performed at ___ minutes?
30 minutes
What does a score of less than or equal to 2 means?
Negative - no indication of current typhoid fever infection
What does a score of 3 means?
Borderline, inconclusive score. Repeat analysis
What does a score of 4 means?
Weak positive - indication of current typhoid fever infection
