Lab Experiment 5 Flashcards
0
Q
Group of bacteria being tested in this experiment?
A
Group A Beta-Streptococci
1
Q
Title of Experiment
A
Antistreptolysin O Titer
2
Q
Examples of extracellular antigens
A
Hemolytic toxin, streptolysin, enzymes hyaluronidase and deoxyribonuclease B
3
Q
Examples of streptococcal infections
A
Rheumatic fever, glomerulonephritis, scarlet fever, tonsilitis
4
Q
Principle of test
A
The latex reagent is a suspension of polystyrene latex particles which allow visual observation of the Ag-Ab reaction. If the antistreptolysin O is present, clear agglutination becomes evident
5
Q
How much of the undiluted serum is to be added?
A
50uL
6
Q
Formula in calculating anti-SLO level in serum sample.
A
ASO titer (IU/ml) = Highest dilution with positive reaction X reagent sensitivity (200IU/ml)
