Lab Experiment 4a and 4b

Question 0

Initial lesion of Syphilis

Answer 0

Chancre

Question 1

Syphilis is a venereal disease caused by a spirochete, the ______________.

Answer 1

Treponema pallidum

Question 2

Two types of Antibodies

Answer 2

Treponemal and Non-treponemal antibody

Question 3

Antibody specific for Treponema pallidum

Answer 3

Treponemal antibody

Question 4

Antibody-like substance formed as a result of the interaction of the treponema with the body tissues

Answer 4

Non-treponemal antibody

Question 5

Two major groups of test for Syphilis

Answer 5

Non-treponemal or Non-specific Reagin tests
And
Specific or Treponemal test

Question 6

Discuss the principle of RPR CARD Test

Answer 6

RPR antigen suspension is a carbon particle cardiolipin antigen which detects “reagin”, an antibody-like subtance present in sera or plasma from syphilitic persons, and occasionally in persons with other acute and chronic conditions

Question 7

What happens when a specimen contains antibody?

Answer 7

Flocculation occurs with a coaggulation of the carbon particles of the RPR antigen

Question 8

How does flocculation looks like in RPR Test?

Answer 8

They appear as black clumps against the white background of the test card. Result can be read macroscopically.

Question 9

Non-reactive specimens appear as what?

Answer 9

An even light-gray color

Question 10

Plasma or serum samples must be free from?

Answer 10

Hemolysis and bacterial contamination

Question 11

If the plasma or serum samples are not used immediately, they must be stored at what temperature?

Answer 11

2 - 8 C

Question 12

If the plasma or serum samples are to be stored more than 24 hours, at what temperature must they be stored?

Answer 12

-20 C

Question 13

Discuss the procedure of RPR CARD Test

Answer 13

Look at manual

Question 14

Discuss the procedure of T. pallidum Hemagglutination Test (4B)

Answer 14

Look at manual

Question 15

What is reagin?

Answer 15

An antibody-like substance present in the plasma or sera from syphilitic persons and occasionally from persons with acute and chronic conditions

Question 16

How many minutes do we rotate the card on the mechanical rotator? And at what rpm?

Answer 16

8 minutes at 100 rpm.

Question 17

How much does one drop of the dispenstir device deliver?

Answer 17

Approximately 0.05 ml/drop

Question 18

What are the titles of Experiments 4a and 4b?

Answer 18

Rapid Plasma Reagin CARD Test (Qualitative Procedure) and Treponema pallidum Hemagglutination Test

Question 19

In Expt 4b, how many uL of diluent must be placed in wells 1, 2 and 3?

Answer 19

10, 25 and 25 uL respectively

Question 20

In Expt 4b, explain number 2 in the procedure.

Answer 20

Add 25 uL of serum sample to well 1. Mix then transfer 25 uL to well 2. Mix then transfer 25 uL to well 3. Mix then discard 25 uL from well 3.

Question 21

In Expt 4b, how many uL of the well resuspended control cells are to be added to wells 2 and 3?

Answer 21

75 uL each

Question 22

In Expt 4b, how many minutes do we leave the microtiter plates before reading the results?

Answer 22

45 - 60 minutes