lab exam two Flashcards
which sugar solution produced the most gas with yeast?
glucose
signs of stress in fish
changes in defecation, eye movement
polar solutes dissolve in
polar solvents
nonpolar solutes dissolve in
nonpolar solvenets
anthocyanins
red, blue and purple pigments
- dissolve in water
chlorophylls
green pigment
- dissolves in less polar solvent (ethanol)
carotenes
- yellow and orange pigment
- dissolves in less polar solvent (ethanol)
xanthophylls
- yellow pigment
- dissolves in less polar solvent (ethanol)
ethanol
highly toxic chemical and alcohol
- drinking will make you ill and possibly blind
what was added to the variegated coleus leaf to determine where the most starch was present?
Lugol’s solution
for every molecule of CO2 produced for fish…
1 molecule NaOH needed to neutralize resulting acid
steps of extracting DNA
- lysing cell using chemical/mechanical means
- addition of salt/detergent solution
- addition of meat tenderizer
- ethanol added
- centrifugation
pathogen
disease agent
pandemic
epidemics that spread from continent to continent
epidemiology
study of disease at the population level
Typhoid Mary
original carrier
phenol red
acid - yellow
- base - magenta/red
Human Genome Project
identifying and mapping 20,000-25,000 genes that define a human being
bioinformatics
field that combines statistics, mathematical modeling, and computer science to analyze biological data
BLAST
basic local alignment search tool
- searches for identical/similar sequences
cladogram
(phylogenetic tree)
- visualization of evolutionary relatedness of species
cladistics
shows relationship between dif organisms base don presence/absence of certain characteristics (derived)
derived characters
unique characteristics of particular group of organisms
what does blending do for DNA extraction
disrupt cell and nuclear membranes
what does salt and detergent do in DNA extraction
break down and emulsify fat and proteins that make up membranes
what does meat tenderizer do
contains proteases (digestive enzymes) that cut away histones that coil DNA
- unwind DNA