Lab Exam 2 Flashcards
E. coli gram + or -
neg
what shape is e coli
rods
can E. coli ferment glucose
yes
catalase results of e. coli
pos
oxidase results of e. coli
neg
Can E. coli ferment lactose
yes
can S. typhimurium ferment lactose
no
what does S. typhimurium produce on selective media
H2S
what does SS agar differentiates bacteria based on
ability to ferment lactose and reduce
sulfur (produce H2S)
sugar is SS agar
lactose
what indicator dye is in SS agar
neutral red
if an organism can ferment lactose on SS agar what color does it turn
pink
what is the pink precipitate in SS agar
bile salts
what does black precipitate on SS agar mean
hydrogen sulfide
(H2S) production
KIA slant; Yellow color = ___ production = ______
acid
fermentation
KIA slants: Red color = _____ products = _____
alkaline
protein catabolism
kia slant: butt is yellow
organism can ferment glucose.
if kia slant is red can the organism ferment lactose
no
Blue Simmons citrate agar, organism can or can’t use citrate?
can
Green Simmons citrate agar, organism can or can’t use citrate?
can’t
indole broth: If indole is present (+), the reagent will turn ____
red
indole broth: If indole is not present (-), the reagent will turn ____
clear
is Streptococcus agalactiae catalase positive or neg
neg
is Streptococcus agalactiae oxidase pos or neg
neg
is Enterococcus faecalis catalase pos or neg
neg
is Enterococcus faecalis oxidase pos or neg
neg
what are streptococci and enterococci shape and arrangement
cocci
chains or pairs
what kind of hemolysis does S. agalactiae have
beta
what kind of hemolysis does enterococcus have
non-hemolytic
true or false: enterococcus are highly antibiotic resistant
true
streptococci are generally _____ to salt while enterococci are generally ___ grow in high salt environments
sensitive
able to
what does a yellow color indicate on a bile esculin slant
negative for esculin hydrolysis
what does a black color indicate on a bile esculin slant
positive for esculin hydrolysis
what does a bacticard strep test for
distinguishes between streptococcus and enterococcus
how fast does bacteria divide
exponentially
how many colonies should be on a plate for standard acceptable range for an accurate
30-300
