lab 9- selective and differential media

Question 1

mannitol Salt agar (MSA) is a good example of ___

Answer 1

differential and selective media

Question 2

what does MSA agar contail

Answer 2

mannitol and pH indicator that indicates the changes of color when mannitol is fermented

Question 3

what is the salt concentration for MSA

Answer 3

7.5

Question 4

what is the only bacteria that can grow on 7.5% salt

Answer 4

staphylococcus

Question 5

what is the pH indicator for MSA

Answer 5

red—-yellow

which indicates the organism is able to ferment mannitol

Question 6

Eosin Methylene Blue (EMB) and MacConkey agars contain substances that

Answer 6

inhibit the growth of gram pos organisms (CV)

Question 7

Eosin Methylene Blue (EMB) and MacConkey

Answer 7

determine lactose fermenting

Question 8

EMB contains what dyes

Answer 8

CV and methylene blue

Question 9

What bacteria was gram + for EMB

Answer 9

S. Aureus

Question 10

coliforms ferment

Answer 10

lactose

Question 11

is e coli gram neg or pos

Answer 11

neg (pink)

Question 12

e coli had what type of color for EMB plate

Answer 12

metalic green

Question 13

S. aureus (Staphylococcus aureus) is

Answer 13

gram pos

Question 14

e coli is or isn’t a lactose fermenter

Answer 14

is a lactose fermenter

Question 15

blood agar is an example of ___

Answer 15

differential media NOT selective

Question 16

s. mitus medium apparence and growth observed

Answer 16

appearence- red/brown
observation- yellow

Question 17

s. pynogenes medium apparence and reaction observed

Answer 17

appearence- red/brown
observation- clear

Question 18

e. faecalis medium apparence and reaction observed

Answer 18

appearence- brown
observation- creamy/white

Question 19

s. mitus reaction and type of hemolysis

Answer 19

reaction- abundant
type- alpha

Question 20

s. pyogenes reaction and type of hemolysis

Answer 20

reaction- complete lysis
type- beta

Question 21

e. faecalis reaction and type of hemolysis