Lab 5- Acid Fast And Spore Staining

Question 1

What is the decolonizing agent used in the acid-fast stain? How is this different from the decolonizing agent used in the gram stain

Answer 1

The decolonizing agent is acid alcohol

The difference is it has a waxy mycolic acid layer

Question 2

What diseases are diagnosed by using the acid fast stain

Answer 2

TB (mycobacterium tuberculosis)

Question 3

What are the steps of acid fast

Answer 3

1. Use aseptic tech, prepare a slide of mycobacterium smagmeatis mixed with a loop full of s. Aureus
2. Air dry then heat fix
3. During air dying we prepare the hot water bath
4. After the heat fix we place the steam
5. Floors the slide with carbon cushion for 5 min. We also dont allow the stain to evaporate
6. Remove the slide from the beaker and place it on the staining tray
   7 cool and gently wash the slide with water
   8 drip acid alcohol on the slide until no more color drain from the smear
   9 wash gently with water
   10 counter stain with methylene blue for 2 min
   11 gently was with water and dry

Question 4

On page 35