Lab 2- aspetic technique Flashcards
Aseptic Transfer is the process of
transferring living microbes from one place to another without contamination of the culture, the sterile medium, or surroundings
aseptic transfer is also known as
sterile technique
How long should the inoculating loop be cooled for before dipping into your test tube?
10 seconds or more
After the glass tube is hovered over the flame, why is important to have the tube be held at an angle?
To reduce the possibility that organisms in the air might fall into the tube
pure culture is __________
a population descended from a single cell
Inoculation
transferring a pure culture of an organism from one medium to another in a process
subculturing
A tiny amount of the microorganism is removed from one medium using an inoculating loop or an inoculating need and transferred or inoculated into or onto fresh medium
subculturing is the process of
transferring microorganisms from one medium to another
Serratia marcescens produces a
red pigment
Serratia marcescens is a _______
gram negative, rod shaped bacteria
How long were Serratia marcescens cultures left incubating?
48 hours
What temperature was Serratia marcescens left to incubate in?
25°C
Media
solid, liquid or semi-solid designed to support the growth of microorganisms or cells
Discuss 2 reasons why the aspect technique is used when studying bacteria ?
to prevent contamination of the bacterial culture with unwanted microorganisms
to protect the researcher from potential harmful bacteria by ensuring a sterile environment during handling and manipulation.
List the advantages of using an inoculating needle compared to using an inoculating loop
Using the needle can allow for more control over how much of the specimen you want on the slide and needles seem to be a lot more durable and sturdy compared to the loop
List two reasons why there may be no growth observed after inoculating a broth or slant with an organism.
It’s possible when there wasn’t enough of the bacteria in your broth/slant or your needle/looop was to hot
sterile culture media
an special uncontaminated medium used in microbiological labs to grow different kinds of microorganisms
inoculated culture
a medium where microorganisms can grow and reproduce
pure culture
a population containing only a single species or stain of bacteria
