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Micro Bio Lab > lab 9 > Flashcards

lab 9 Flashcards

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Biology 101 flashcards
1
Q

What is the diameter of viruses?

A

between 10 and 300nm

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2
Q

What is a virus?

A

small infectious agent that replicates only inside living cells of other organisms. it infects animals, plants, microorganisms including bacteria

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3
Q

what is a viron ?

A

free viral particles (outside the cell or in the process of infecting a cell).

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4
Q

what is genetic material?

A

DNA or RNA

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5
Q

What is capsid?

A

protein coat

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6
Q

what is envelope of lipids?

A

surrounding the capsid

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7
Q

what are bacteriophages?

A

viruses that infect bacteria

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8
Q

what is procapsid?

A

capsid in precursor form

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9
Q

What is a concatomer?

A

a long continuous DNA molecule that contains multiple copies of the same DNA sequences linked in series

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10
Q

what happens if DNA is too large ? what modifys it?

A

it wont fit into the procapsid. an enzyme cuts the DNA.

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11
Q

what is Lytic virus P1?

A

bacteriophage dsDNA that infects E. coli and other bacteria

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12
Q

what is lysogenic cycle?

A

the phage genome exists as a plasmid in the bacterium

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13
Q

What is the first stage of the lytic cycle?

A

Attachment: phage attaches to host cell

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14
Q

what is the second stage of the cycle ?

A

Penetration: phage penetrates host cell and injects its DNA

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15
Q

what is the third stage?

A

biosynthesis: phage DNA directs synthesis of viral components by the host cell.

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16
Q

the fourth stage?

A

maturation: assembly of phage particles called virions.

17
Q

the fifth stage ?

A

phage lysozyme breaks cell wall due high number of virions produced.

18
Q

what is a prophage?

A

bacteriophage genome inserted and integrated into the circular bacterial DNA chromosome or existing as a plasmid

19
Q

Rolling circle replication

A

Process of unidirectional nucleic acid replication that can rapidly synthesize multiple copies of circular molecules of DNA.

20
Q

What is a capsid ? (protein coat)

A

protection of nucleic acid from destruction by DNAses

21
Q

Nucleic acid core

A

phage genome carrying genetic information necessary for replication of new phage particles.

22
Q

Spiral protein sheath

A

retracts so that nucleic acid can pass from capsid into host cell’s cytoplasm

23
Q

End plate and tail fibers-

A

attachment of phage to specific receptor sites on a susceptible host’s cell wall.

Micro Bio Lab (9 decks)

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