lab 4 Flashcards

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1
Q

selective media

A

will inhibit the growth of different bacteria to allow for one specific type to grow

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2
Q

differential media

A

will allow growth of multiple types of bacteria, but they will look different on a plate

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3
Q

what is an example of both selective and differential

A

Mannitol Salt Agar

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4
Q

How is Mannitol Salt Agar selective ?

A

due to high salt concentration (7.5-10%) and used for the ISOLATION of gram + bacteria, pathogenic Staphylococci and non pathogenic Staphylococci. PREVENTS GROWTH OF GRAM -

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5
Q

How is Mannitol Salt Agar differential ?

A

it contains mannitol that can be fermented and PHENOL RED.

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6
Q

What color indicates fermentation ?

A

acidic and yellow

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7
Q

What is a sign of no fermentation ?

A

red color

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8
Q

How is MacConkey Agar selective ?

A

Bile salts isolate gram (-) bacteria by inhibiting gram (+) bacteria.

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9
Q

How is MacConkey Agar differential?

A

Lactose is used to differentiate bacteria based on their ability to ferment

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10
Q

In MacConkey Agar, what color indicates fermentation of lactose ?

A

pink

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11
Q

What color is it when fermentation does not occur ?

A

pale color

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12
Q

How is Vogel Johnson Agar selective ?

A

Selective for gram (+) by use of LiCl, tellurite, and glycine content

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13
Q

How is Vogel Johnson Agar differential?

A

Contains mannitol and phenol red indicator for differentiation (just like the
mannitol salt agar)

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14
Q

In Vogel Johnson Agar, which color indicates fermentation?

A

yellow

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15
Q

What can bacteria do differently in Vogel Johnson Agar>

A

Bacteria can reduce tellurite to tellurium = black

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16
Q

How is Eosin Methylene Blue selective?

A

Methylene blue isolates gram (-) by inhibiting gram (+) bacteria

17
Q

How is Eosin Methylene Blue differential?

A

Lactose and sucrose fermentation is used to differentiate

18
Q

How is Brilliant Green Agar selective?

A

isolates gram (-) bacteria

19
Q

How is Brilliant Green Agar differential?

A

Lactose and sucrose fermentation is used to differentiate bacteria.

20
Q

What color indicates fermentation?

A

yellow

21
Q

what color indicates no fermentation in brilliant green agar ?

A

red

22
Q

How is Hektoen Enteric Agar selective ?

A

bile salts isolate gram (-) bacteria by inhibiting gram (+) bacteria (

23
Q

how is Hektoen Enteric Agar differential?

A

used to differentiate between Shigella and Salmonella bacteria

24
Q

what color indicates lactose fermentation?

A

orange

25
Q

What creates black to greenish colonies ?

A

Hydrogen sulfide producers or Salmonella

26
Q

what is e coli

A

the lactose fermenters that create pink to orange colonies

27
Q

Which involve only gram-positive (+) isolation?

A

-Mannitol Salt Agar
- Vogel Johnson Aga