Lab 9 Flashcards
Van’t Hoff equation function
estimates Q10 when measurements of rate are not taken at an even 10 degrees apart
Q10 meaning
the ratio of the metabolic rate at one temperature to the rate at a temperature 10 degree celsius lower
Acclimation:
Experimentally altering environmental temperature to induce physiological compensations
Acclimatization:
Seasonal physiological
compensations to changes in temperature
Basal metabolic rate =
endotherm’s (mammals) resting
metabolic rate at thermal neutral zone
Standard metabolic rate =
ectotherm’s resting
metabolic rate at a particular temperature within its
normal range
Resting Metabolic Rate (RMR) =
energy expenditure
at rest
In order to measure resting metabolic rate the
animal should be controlled or assumed ( 5 THINGS) :
– Inactive
– Under no stress (illness or injury)
– Fasted (not have eaten food recently)
– Normal physiologically (not pregnant; not hibernating)
– Be at an appropriate temperature
calorie =
amount of heat needed to raise 1g of
water by 1oC
O2 consumed/time (ml O2/hr) can be used to
estimate energy expenditure
(calories burned or heat production)
Metabolic rate =
energy expenditure/time
define respirometry
technique used for obtaining estimates of the rates of metabolism via measure of oxygen consumption
respirometer function
determine oxygen consumption
name the invertebrate ectotherm used
Helisoma trivolvis ( pond snail)
name the freshwater vertebrate ectotherm used
Danio rerio (zebrafish)
function of parafilm
to seal chamber to avoid air leaks
what formula is used to calculate the volume of oxygen in the chamber at each sampling time
vol O2 = PO2 * V * aO2
Vol O2 units and meaning
volume of oxygen in mL
PO2 meaning and units
pressure of oxygen in torr/mmHg
V meaning and unit in equation
volume of water in respirometer in Liters
aO2 meaning and units
oxygen solubility coefficient for water in mL 02/ LH2O/torr
This equation is used for:
V_O2 = ( Pi - Pe) * V * aO2
what does each variable mean?
conver O2 PRESSURE TO O2 consumption
VO2 = volume of oxygen
Pi = partial pressure of 02 in incurrent water
Pe = partial pressure of O2 in excurrent water
define differential respirometry
the difference in inflow and outflow of water to calculate the amount of oxygen utilized for animal respiration
thermal compensation
physiological systems increase activity to maintain a stable function in spite of temp changes.
100% oxygen on labchart is =
160 mmHg
at what temperature will all measurements be conducted
22 degree C
