Lab 8

Question 1

exonuclease

Answer 1

degrades remaining primers by removing nucleotides from the primers in a 3’ to 5’ direction

Question 2

2. Know the general procedural details for gel electrophoresis and PCR clean up by the Exo-AP
   method

Answer 2

1. Load samples into wells
2. Run gel at ~100 volts for however long you need to (45 mins?)

Question 3

phosphatase

Answer 3

Inactivates dNTPs by removing 5’ phosphate on the dNTPs. (Can’t incorporate themselveds on DNA string anymore

Question 4

next- generation sequencing technologies

Answer 4

the sequencing methods commonly used to sequence whole genomes