Lab 5

Question 1

One microliter is equal to

Answer 1

0.001 milliliter.

Question 2

A critical technique used in molecular genetic analysis is a restriction digest, which creates _____________ in DNA.

Answer 2

double-stranded cuts

Question 3

A restriction digestion is carried out by special enzymes called _______________, or oftentimes called _____________________.

Answer 3

restriction endonucleases, restriction enzymes

Question 4

Many different types of restriction enzymes have been identified, but the most widely used are the __________________, which recognize specific nucleotide sequences and make _______________ within those sequences.

Answer 4

Type II restriction enzymes, double-stranded cuts

Question 5

The restriction sites recognized by these enzymes are _________________, which are read the same in both directions when looking at the top and bottom strands of DNA (5′ to 3′).

Answer 5

palindromic sequences

Question 6

Restriction enzymes were first isolated from bacteria, where they are used as a ___________. To prevent their own DNA from being digested, bacteria ________________________

Answer 6

defense mechanism against viruses that infect the bacteria, methylate their restriction sites such that it is not recognized by the enzyme

Question 7

Restriction enzymes that create blunt cuts do so because ______________

Answer 7

the cuts are made at the same location on both strands.

Question 8

HaeIII restriction site

Answer 8

GG|CC
CC|GG

Question 9

EcoRI restriction site

Answer 9

G|ATTC
CTTA|G