lab 7 - energy flow i

Question 1

what is the main objective of the cellular respiration experiment?

Answer 1

study the role of succinate dehydrogenase in the citric acid cycle and its ability to reduce FAD

Question 2

what is the objective of the photosynthesis experiment?

Answer 2

investigate how light drives the reduction of NADP+ to NADPH

Question 3

what happens to blue dye when succinate dehydrogenase is active?

Answer 3

blue dye will be reduced and change from blue to clear

Question 4

is color change of the blue dye the dependent or independent variable?

Answer 4

dependent variable

Question 5

is exposure of chloroplasts to light the dependent or independent variable?

Answer 5

independent variable

Question 6

true or false - blue dye is an electron acceptor

Answer 6

true

Question 7

what role does the blue dye play in both experiements?

Answer 7

it acts as an electron acceptor to monitor the transfer of electrons (reduction reaction)

Question 8

what enzyme is studied in the cellular respiration experiment?

Answer 8

succinate dehydrogenase

Question 9

what is the function of succinate dehydrogenase?

Answer 9

an enzyme that helps turn succinate into fumarate, removes hydrogen, the removed hydrogen is passed to a carrier molecule called FAD, which gets turned into FADH2

Question 10

which organelle is involved in cellular respiration?

Answer 10

mitochondria

Question 11

if light provides energy for the reduction of NADP+ to NADPH, what happens to the blue dye?

Answer 11

blue dye will turn clear as it accepts electrons

Question 12

what is oxidation?

Answer 12

loss of electrons or hydrogen

Question 13

what is reduction?

Answer 13

gain of electrons or hydrogen

Question 14

what is the role of blue dye in cellular respiration?

Answer 14

blue dye turns colourless as cells consume oxygen (used to track progress and show changes)

Question 15

what is the alternate hypothesis?

Answer 15

the blue dye will change colour when succinate is present