lab 5 -proteins i Flashcards
1
Q
what does the biuret test determine?
A
the presence of proteins, indicated by a color change from blue to purple based on protein concentration
2
Q
what is the dilution formula used in lab 5?
A
C1V1=C2V2
3
Q
what data is plotted for the standard curve?
A
absorbance (y-axis) vs protein concentration (x-axis)
4
Q
what is the independent variable?
A
protein concentration (mg/ml)
5
Q
what is the dependent variable?
A
absorbance at 540nm
6
Q
what does calibrating the spectrophotometer mean?
A
ensures that only the absorbance of the protein is measured
7
Q
what is the blank cuvette filled with?
A
water
8
Q
why do we need a blank cuvette to calibrate the spectrophotometer?
A
to eliminate absorbance from the water, buret reagent, or the cuvette itself
